Invitrogen

VGLUT1 Polyclonal Antibody

VGLUT1 Antibody in Immunocytochemistry (ICC/IF)

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VGLUT1 Antibody (48-2400) in ICC/IF

Immunofluorescence analysis of VGLUT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VGLUT1 Rabbit Polyclonal Antibody (Product # 48-2400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More

VGLUT1 Antibody in Immunohistochemistry (IHC)

VGLUT1 Antibody in Immunohistochemistry - Free Floating (IHC (Free))

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Product Details

48-2400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

2-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

View 8 publications 8 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the internal region of the mouse VGLUT1 protein (Accession# NP_892038, Q3TXX4), which is 93% homologous to human and rat.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533843

Product Specific Information

This antibody is specific for the VGLUT1 protein. On western blots, it identifies the target band at ~55 kDa. Reactivity has been confirmed with mouse brain homogenates by western blotting, with mouse hippocampus frozen sections by immunohistochemistry, and in immunofluorescence analysis with human glutamate neurons derived from iPSCs.

Target Information

A Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Efficient generation of brain organoids using magnetized gold nanoparticles.

Scientific reports

Kim H,Lee YJ,Kwon Y,Kim J

48-2400 was used in Immunohistochemistry to present a method for effectively generating cerebral organoids from human induced pluripotent stem cells (hiPSCs) using electromagnetic gold nanoparticles (AuNPs).

Fri Dec 01 00:00:00 UTC 2023

Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics.

EBioMedicine

Rowe AA,Chen X,Nettesheim ER,Issioui Y,Dong T,Hu Y,Messahel S,Kayani SN,Gray SJ,Wert KJ

48-2400 was used in Immunohistochemistry to find that this preclinical model shows signs of photoreceptor to bipolar synaptic defects early, and displays rod-cone dystrophy with late loss of bipolar cells.

Tue Nov 01 00:00:00 UTC 2022

Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics.

EBioMedicine

Rowe AA,Chen X,Nettesheim ER,Issioui Y,Dong T,Hu Y,Messahel S,Kayani SN,Gray SJ,Wert KJ

Tue Nov 01 00:00:00 UTC 2022

Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics.

EBioMedicine

Rowe AA,Chen X,Nettesheim ER,Issioui Y,Dong T,Hu Y,Messahel S,Kayani SN,Gray SJ,Wert KJ

Tue Nov 01 00:00:00 UTC 2022

Synaptosome microRNAs regulate synapse functions in Alzheimer's disease.

NPJ genomic medicine

Kumar S,Orlov E,Gowda P,Bose C,Swerdlow RH,Lahiri DK,Reddy PH

48-2400 was used in Western Blotting to describe synaptic versus cytosolic miRNAs in Alzheimer's disease and their significance in synapse function.

Mon Aug 08 00:00:00 UTC 2022

Synaptosome microRNAs regulate synapse functions in Alzheimer's disease.

NPJ genomic medicine

Kumar S,Orlov E,Gowda P,Bose C,Swerdlow RH,Lahiri DK,Reddy PH

48-2400 was used in Western Blotting to describe synaptic versus cytosolic miRNAs in Alzheimer's disease and their significance in synapse function.

Mon Aug 08 00:00:00 UTC 2022

Long-Term Hyperglycemia Causes Depressive Behaviors in Mice with Hypoactive Glutamatergic Activity in the Medial Prefrontal Cortex, Which Is Not Reversed by Insulin Treatment.

Cells

Baek JH,Son H,Kang JS,Yoo DY,Chung HJ,Lee DK,Kim HJ

48-2400 was used in Immunohistochemistry-immunofluorescence to suggest that hypoactive glutamatergic activity in the mPFC is involved in diabetic-associated depressive behaviors, and it is difficult to cure with glycemic regulation alone.

Mon Dec 12 00:00:00 UTC 2022

Long-Term Hyperglycemia Causes Depressive Behaviors in Mice with Hypoactive Glutamatergic Activity in the Medial Prefrontal Cortex, Which Is Not Reversed by Insulin Treatment.

Cells

Baek JH,Son H,Kang JS,Yoo DY,Chung HJ,Lee DK,Kim HJ

Mon Dec 12 00:00:00 UTC 2022

A microengineered Brain-Chip to model neuroinflammation in humans.

iScience

Pediaditakis I,Kodella KR,Manatakis DV,Le CY,Barthakur S,Sorets A,Gravanis A,Ewart L,Rubin LL,Manolakos ES,Hinojosa CD,Karalis K

48-2400 was used in Immunocytochemistry to present a human organotypic microphysiological system (MPS) that includes endothelial-like cells, pericytes, glia, and cortical neurons and maintains brain and blood-brain barrier permeability at in vivo relevant levels.

Fri Aug 19 00:00:00 UTC 2022

Primary brain cell infection by Toxoplasma gondii reveals the extent and dynamics of parasite differentiation and its impact on neuron biology.

Open biology

Mouveaux T,Roger E,Gueye A,Eysert F,Huot L,Grenier-Boley B,Lambert JC,Gissot M

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Western Blot

Fri Oct 01 00:00:00 UTC 2021

Olivocochlear Changes Associated With Aging Predominantly Affect the Medial Olivocochlear System.

Frontiers in neuroscience

Vicencio-Jimenez S,Weinberg MM,Bucci-Mansilla G,Lauer AM

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Immunohistochemistry

Wed Jan 05 00:00:00 UTC 2022

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Scientific reports

Jones B,Balasubramaniam M,Lebowitz JJ,Taylor A,Villalta F,Khoshbouei H,Grueter C,Grueter B,Dash C,Pandhare J

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Western Blot

Thu Jan 14 00:00:00 UTC 2021

Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells.

Cells

Soni A,Klütsch D,Hu X,Houtman J,Rund N,McCloskey A,Mertens J,Schafer ST,Amin H,Toda T

48-2400 was used in Immunocytochemistry to demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks.

Mon Jul 26 00:00:00 UTC 2021

Direct conversion of human fibroblasts into dopaminergic neuron-like cells using small molecules and protein factors.

Military Medical Research

Qin H,Zhao AD,Sun ML,Ma K,Fu XB

48-2400 was used in Immunocytochemistry to develope a chemical cocktail of small molecules and protein factors to convert human fibroblasts into DA neuron-like cells without passing through a neural progenitor/stem cell intermediate stage.

Sun Nov 01 00:00:00 UTC 2020

Direct conversion of human fibroblasts into dopaminergic neuron-like cells using small molecules and protein factors.

Military Medical Research

Qin H,Zhao AD,Sun ML,Ma K,Fu XB

Sun Nov 01 00:00:00 UTC 2020

Astrocytic Ephrin-B1 Controls Excitatory-Inhibitory Balance in Developing Hippocampus.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nguyen AQ,Sutley S,Koeppen J,Mina K,Woodruff S,Hanna S,Vengala A,Hickmott PW,Obenaus A,Ethell IM

48-2400 was used in Immunohistochemistry to examine whether the loss or overexpression of the membrane-bound ephrin-B1 in astrocytes during postnatal day 14-28 period would affect synapse formation and maturation in the developing hippocampus.

Wed Sep 02 00:00:00 UTC 2020

Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory.

Frontiers in synaptic neuroscience

Nguyen AQ,Koeppen J,Woodruff S,Mina K,Figueroa Z,Ethell IM

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Immunohistochemistry

Thu Apr 25 00:00:00 UTC 2024

Small molecular compounds efficiently convert human fibroblasts directly into neurons.

Molecular medicine reports

Yang J,Cao H,Guo S,Zhu H,Tao H,Zhang L,Chen Z,Sun T,Chi S,Hu Q

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex.

BMC neuroscience

Park SS,Lee DH,Lee SM,Lee CH,Kim SY

48-2400 was used in Western Blotting to investigate the changes in molecules related to perineuronal nets (PNNs) and synaptic transporters in the primary auditory cortices of rats with noise-induced hearing loss.

Sat Apr 25 00:00:00 UTC 2020

Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex.

BMC neuroscience

Park SS,Lee DH,Lee SM,Lee CH,Kim SY

Sat Apr 25 00:00:00 UTC 2020

Striosomes Mediate Value-Based Learning Vulnerable in Age and a Huntington's Disease Model.

Cell

Friedman A,Hueske E,Drammis SM,Toro Arana SE,Nelson ED,Carter CW,Delcasso S,Rodriguez RX,Lutwak H,DiMarco KS,Zhang Q,Rakocevic LI,Hu D,Xiong JK,Zhao J,Gibb LG,Yoshida T,Siciliano CA,Diefenbach TJ,Ramakrishnan C,Deisseroth K,Graybiel AM

48-2400 was used in Immunohistochemistry-immunofluorescence to study circuit connectivity and function of the striosome compartment of the striatum are critical for learning valence-based responses using engineered mice.

Thu Nov 12 00:00:00 UTC 2020

Protein kinase N1 critically regulates cerebellar development and long-term function.

The Journal of clinical investigation

zur Nedden S,Eith R,Schwarzer C,Zanetti L,Seitter H,Fresser F,Koschak A,Cameron AJ,Parker PJ,Baier G,Baier-Bitterlich G

48-2400 was used in Western Blot to identify protein kinase N1 as a novel key player in fine-tuning the balance between axonal outgrowth and presynaptic differentiation in the parallel fibre-forming cerebellar granule cells.

Tue May 01 00:00:00 UTC 2018

3D Culture Method for Alzheimer's Disease Modeling Reveals Interleukin-4 Rescues Aβ42-Induced Loss of Human Neural Stem Cell Plasticity.

Developmental cell

Papadimitriou C,Celikkaya H,Cosacak MI,Mashkaryan V,Bray L,Bhattarai P,Brandt K,Hollak H,Chen X,He S,Antos CL,Lin W,Thomas AK,Dahl A,Kurth T,Friedrichs J,Zhang Y,Freudenberg U,Werner C,Kizil C

48-2400 was used in Immunocytochemistry-immunoflourescence to conclude that our culture system enables a reductionist investigation of regulation of human NSC plasticity for the identification of potential therapeutic targets for intervention in AD.

Mon Jul 02 00:00:00 UTC 2018

Functional Consequences of Synapse Remodeling Following Astrocyte-Specific Regulation of Ephrin-B1 in the Adult Hippocampus.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Koeppen J,Nguyen AQ,Nikolakopoulou AM,Garcia M,Hanna S,Woodruff S,Figueroa Z,Obenaus A,Ethell IM

48-2400 was used in Immunohistochemistry to examine the role of astrocytic ephrin-B1 as a regulator of synaptogenesis in the murine adult hippocampus and learning behaviours.

Wed Jun 20 00:00:00 UTC 2018

Central Compensation in Auditory Brainstem after Damaging Noise Exposure.

eNeuro

Schrode KM,Muniak MA,Kim YH,Lauer AM

48-2400 was used in Immunohistochemistry to investigate the link between noise exposure, increases in central gain, synaptic reorganisation, and auditory function.

Fri Mar 08 00:00:00 UTC 2019

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Neurovascular Interaction Promotes the Morphological and Functional Maturation of Cortical Neurons.

Frontiers in cellular neuroscience

Wu KW,Mo JL,Kou ZW,Liu Q,Lv LL,Lei Y,Sun FY

48-2400 was used in Immunocytochemistry to provide novel aspects of endothelium-neuron interactions and novel mechanism of neurovascular crosstalk.

Wed Sep 30 00:00:00 UTC 2020

Astrocytic Ephrin-B1 Regulates Synapse Remodeling Following Traumatic Brain Injury.

ASN neuro

Nikolakopoulou AM,Koeppen J,Garcia M,Leish J,Obenaus A,Ethell IM

Published figure using VGLUT1 polyclonal antibody (Product # 48-2400) in Immunofluorescence

Tue Dec 13 00:00:00 UTC 2016

Auditory nerve synapses persist in ventral cochlear nucleus long after loss of acoustic input in mice with early-onset progressive hearing loss.

Brain research

McGuire B,Fiorillo B,Ryugo DK,Lauer AM

48-2400 was used in immunohistochemistry - free floating to report the gross morphology of the ventral cochlear nucleus and auditory nerve synapse integrity in mice with early-onset progressive sensorineural hearing loss

Fri Apr 24 00:00:00 UTC 2015

Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sidhu H,Dansie LE,Hickmott PW,Ethell DW,Ethell IM

48-2400 was used in immunocytochemistry to investigate the involvement of matrix metalloproteinase 9 in the phenotype of fragile X syndrome.

Wed Jul 23 00:00:00 UTC 2014

Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.

BioResearch open access

Gunewardene N,Bergen NV,Crombie D,Needham K,Dottori M,Nayagam BA

48-2400 was used in immunocytochemistry to study how to differentiate hiPSCs into a neurosensory lineage.

Fri Aug 01 00:00:00 UTC 2014

Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.

BioResearch open access

Gunewardene N,Bergen NV,Crombie D,Needham K,Dottori M,Nayagam BA

48-2400 was used in immunocytochemistry to study how to differentiate hiPSCs into a neurosensory lineage.

Fri Aug 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Brain-specific Na(+)-dependent inorganic phosphate cotransporter; brain-specific Na-dependent inorganic phosphate cotransporter; SLC17A1; SLC17A1/VGLUT1; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; solute carrier family 17 (vesicular glutamate transporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGlU1; VGLUT 1; VGluT1

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Gene Aliases: 2900052E22Rik; AI851913; BNPI; SLC17A7; VGLUT1

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UniProt ID: (Human) Q9P2U7, (Rat) Q62634, (Mouse) Q3TXX4

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Entrez Gene ID: (Human) 57030, (Rat) 116638, (Mouse) 72961

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Function(s)

L-glutamate transmembrane transporter activity inorganic phosphate transmembrane transporter activity extracellular-glutamate-gated chloride channel activity sodium:inorganic phosphate symporter activity sodium-dependent phosphate transmembrane transporter activity symporter activity

Process(es)

phosphate ion transport neurotransmitter transport chemical synaptic transmission long-term memory synaptic transmission, glutamatergic phosphate ion transmembrane transport sodium ion transmembrane transport sequestering of neurotransmitter sodium-dependent phosphate transport L-glutamate import excitatory postsynaptic potential synaptic vesicle lumen acidification neural retina development ion transport brain development glutamate secretion L-glutamate transmembrane transport chloride transmembrane transport transport sodium ion transport response to stimulus transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VGLUT1 Polyclonal Antibody

VGLUT1 Antibody (48-2400) in ICC/IF

Product Details

48-2400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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