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Invitrogen
VHL Polyclonal Antibody
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FIGURE: 1 / 7
VHL Antibody (PA5-13487) in ICC/IF
Immunocytochemistry analysis of VHL in Hela (Human Cervical epithelial adenocarcinoma cell line) cells. Samples were incubated with VHL polyclonal antibody (Product # PA5-13487) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear ... View More
Product Details
PA5-13487
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human VHL
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2215945
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elongin binding protein; Protein G7; pVHL; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1; Vhlh
UniProt ID: (Human) P40337, (Mouse) P40338
Entrez Gene ID: (Human) 7428, (Mouse) 22346
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
regulation of transcription, DNA-templated
proteolysis
negative regulation of cell proliferation
protein ubiquitination
negative regulation of apoptotic process
positive regulation of cell differentiation
positive regulation of transcription, DNA-templated
protein stabilization
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
angiogenesis
response to hypoxia
type B pancreatic cell differentiation
pancreatic A cell differentiation
NLS-bearing protein import into nucleus
proteasomal protein catabolic process
protein transport
protein catabolic process
neuron differentiation
extracellular matrix organization
regulation of catecholamine metabolic process
blood vessel endothelial cell migration
negative regulation of endothelial cell differentiation
protein heterooligomerization
regulation of thymocyte apoptotic process
negative regulation of thymocyte apoptotic process
negative regulation of hypoxia-inducible factor-1alpha signaling pathway
regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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