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Invitrogen
VHL Recombinant Rabbit Monoclonal Antibody (5A8A9)
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FIGURE: 1 / 1
VHL Antibody (MA5-55352) in WB
Western Blot analysis of various lysates using VHL Recombinant Monoclonal Antibody (Product # MA5-55352) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
Product Details
MA5-55352
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5A8A9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Von Hippel Lindau/VHL.
Conjugate
Form
Liquid
Concentration
1.42 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667593
Product Specific Information
Immunogen Sequence: TLPPGTGRRI HSYRGHLWLF RDAGTHDGLL VNQTELFVPS LNVDGQPIFA NITLPVYTLK ERCLQVVRSL VKPENYRRLD IVRSLYEDLE DHPNVQKDLE R
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: elongin binding protein; Protein G7; pVHL; VHL19; VHLp18(MEA); Von Hippel Lindau; von hippel-lindau; von Hippel-Lindau disease tumor suppressor; von Hippel-Lindau syndrome homolog; von Hippel-Lindau syndrome protein homolog; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
Gene Aliases: HRCA1; pVHL; RCA1; VHL; VHL1; Vhlh
UniProt ID: (Human) P40337, (Mouse) P40338, (Rat) Q64259
Entrez Gene ID: (Human) 7428, (Mouse) 22346, (Rat) 24874
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
regulation of transcription, DNA-templated
proteolysis
negative regulation of cell proliferation
protein ubiquitination
negative regulation of apoptotic process
positive regulation of cell differentiation
positive regulation of transcription, DNA-templated
protein stabilization
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
angiogenesis
response to hypoxia
type B pancreatic cell differentiation
pancreatic A cell differentiation
NLS-bearing protein import into nucleus
proteasomal protein catabolic process
protein transport
protein catabolic process
neuron differentiation
extracellular matrix organization
regulation of catecholamine metabolic process
blood vessel endothelial cell migration
negative regulation of endothelial cell differentiation
protein heterooligomerization
regulation of thymocyte apoptotic process
negative regulation of thymocyte apoptotic process
negative regulation of hypoxia-inducible factor-1alpha signaling pathway
regulation of apoptotic signaling pathway
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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