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VNN1 Antibody (PA5-95389) in WB
Western blot analysis of VNN1 in, Lane 1: SKOV3 whole cell lysates, Lane 2: HELA whole cell lysates lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with VNN1 Polyclonal Antibody ... View More
Product Details
PA5-95389
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human VNN1 recombinant protein (Position: Q22-K192).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807192
Product Specific Information
Human VNN1 shares 82.2% amino acid (aa) sequence identity with mouse VNN1.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: SKOV3 whole cell, HELA whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Hematopoietic precursor cells migrate to the thymus, where they differentiate into mature T lymphocytes. GPI-anchored vanin-1 protein regulates the late adhesion steps of thymus homing of bone marrow precursor cells. Vanin-1 is ubiquitously expressed as a pantetheinase enzyme and catalyzes the hydrolysis of pantetheine for vitamin B5 recycling. The hydrolytic activity of vanin-1 generates the potent antioxidant cysteamine as a metabolite. As a membrane bound pantetheinase, vanin-1 provides the main source of cysteamine under normal physiological conditions. In mice, vanin-1 is expressed specifically in male Sertoli cells of the developing testis, where it aids in cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Pantetheinase; Pantetheine hydrolase; Tiff66; Vanin-1; Vanin1; vannin 1; Vascular non-inflammatory molecule 1
Gene Aliases: HDLCQ8; Tiff66; VNN1
UniProt ID: (Human) O95497
Entrez Gene ID: (Human) 8876
acute inflammatory response
chronic inflammatory response
biotin metabolic process
nitrogen compound metabolic process
movement of cell or subcellular component
inflammatory response
response to oxidative stress
central nervous system development
pantothenate metabolic process
single organismal cell-cell adhesion
positive regulation of T cell differentiation in thymus
innate immune response
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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