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VPARP Antibody (PA5-100748) in ICC/IF
Immunofluorescent analysis of VPARP in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and VPARP polyclonal antibody (Product # PA5-100748) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100748
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PARP4(Accession Q9UKK3), corresponding to amino acid residues F1364-L1414.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850251
Product Specific Information
Antibody detects endogenous levels of total PARP4.
Target Information
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 193 kDa vault protein; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; ARTD4; H5 proline-rich; I-alpha-I-related; PARP-4; PARP-related; PARP-related/IalphaI-related H5/proline-rich; PH5P; poly (ADP-ribose) polymerase family, member 4; Poly [ADP-ribose] polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; Protein mono-ADP-ribosyltransferase PARP4; Vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C; VPARP
Gene Aliases: ADPRTL1; ARTD4; C030027K23Rik; E230037B21Rik; Gm743; KIAA0177; p193; PARP-4; PARP4; PARPL; PH5P; VAULT3; VPARP; VWA5C
UniProt ID: (Human) Q9UKK3, (Mouse) E9PYK3
Entrez Gene ID: (Human) 143, (Mouse) 328417
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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