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FIGURE: 1 / 1
VPARP Antibody (PA5-120485) in WB
Western blot analysis of VPARP in extracts of various cell lines. Samples were incubated with VPARP Polyclonal antibody (Product # PA5-120485) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120485
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP4 (NP_006428.2).
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914057
Product Specific Information
Positive test controls include: HeLa, Mouse heart. The target is usually found in the following locations: Cytoplasm, Nucleus, cytoskeleton, spindle.
Immunogen sequence: MVMGIFANCI FCLKVKYLPQ QQKKKLQTDI KENGGKFSFS LNPQCTHIIL DNADVLSQYQ LNSIQKNHVH IANPDFIWKS IREKRLLDVK NYDPYKPLDI TPPPDQKASS SEVKTEGLCP DSATEEEDTV ELTEFGMQNV EIPHLPQDFE VAKYNTLEKV GMEGGQEAVV VELQCSRDSR DCPFLISSHF LLDDGMETRR QFAIKKTSED ASEYFENYIE ELKKQGFLLR EHFTPEATQL ASEQLQALLL EEVMNSSTLS QEVSDLVEMI WAEALGHLEH MLLKPVNRIS LNDVSKAEGI
Target Information
This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 193 kDa vault protein; ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; ARTD4; H5 proline-rich; I-alpha-I-related; PARP-4; PARP-related; PARP-related/IalphaI-related H5/proline-rich; PH5P; poly (ADP-ribose) polymerase family, member 4; poly (ADP-ribose) polymerase)-like 1; Poly [ADP-ribose] polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; Protein mono-ADP-ribosyltransferase PARP4; Vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C; VPARP
Gene Aliases: ADPRTL1; ARTD4; C030027K23Rik; E230037B21Rik; Gm743; KIAA0177; p193; PARP-4; PARP4; PARPL; PH5P; VAULT3; VPARP; VWA5C
UniProt ID: (Human) Q9UKK3, (Mouse) E9PYK3
Entrez Gene ID: (Human) 143, (Rat) 361046, (Mouse) 328417
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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