Proteintech

VPS11 Polyclonal Antibody

VPS11 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

VPS11 Antibody (19140-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using VPS11 antibody 19140-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

VPS11 Antibody in Immunoprecipitation (IP)

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Product Details

19140-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS11 Fusion Protein Ag6227 (689-941 aa encoded by BC065563)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FQQIMHYHM QHEQYRQVIS VCERHGEQDP SLWEQALSYF ARKEEDCKEY VAAVLKHIEN KNLMPPLLVV QTLAHNSTAT LSVIRDYLVQ KLQKQSQQIA QDELRVRRYR EETTRIRQEI QELKASPKIF QKTKCSICNS ALELPSVHFL CGHSFHQHCF ESYSESDADC PTCLPENRKV MDMIRAQEQK RDLHDQFQHQ LRCSNDSFSV IADYFGRGVF NKLTLLTDPP TARLTSSLEA GLQRDLLMHS RRGT (689-941 aa encoded by B C065563)

Target Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes.

Autophagy

Zheng W,Chen Q,Liu H,Zeng L,Zhou Y,Liu X,Bai Y,Zhang J,Pan Y,Shao C

Wed Mar 01 00:00:00 UTC 2023

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.

Journal of molecular biology

Hunter MR,Hesketh GG,Benedyk TH,Gingras AC,Graham SC

Fri Jul 06 00:00:00 UTC 2018

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.

Journal of molecular biology

Hunter MR,Hesketh GG,Benedyk TH,Gingras AC,Graham SC

Fri Jul 06 00:00:00 UTC 2018

The topology of the lymphotoxin β receptor that accumulates upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

Journal of cell science

Banach-Orłowska M,Jastrzębski K,Cendrowski J,Maksymowicz M,Wojciechowska K,Korostyński M,Moreau D,Gruenberg J,Miaczynska M

Wed Nov 21 00:00:00 UTC 2018

The topology of the lymphotoxin β receptor that accumulates upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

Journal of cell science

Banach-Orłowska M,Jastrzębski K,Cendrowski J,Maksymowicz M,Wojciechowska K,Korostyński M,Moreau D,Gruenberg J,Miaczynska M

Wed Nov 21 00:00:00 UTC 2018

The topology of the lymphotoxin β receptor that accumulates upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

Journal of cell science

Banach-Orłowska M,Jastrzębski K,Cendrowski J,Maksymowicz M,Wojciechowska K,Korostyński M,Moreau D,Gruenberg J,Miaczynska M

Wed Nov 21 00:00:00 UTC 2018

The topology of the lymphotoxin β receptor that accumulates upon endolysosomal dysfunction dictates the NF-κB signaling outcome.

Journal of cell science

Banach-Orłowska M,Jastrzębski K,Cendrowski J,Maksymowicz M,Wojciechowska K,Korostyński M,Moreau D,Gruenberg J,Miaczynska M

Wed Nov 21 00:00:00 UTC 2018

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

The Biochemical journal

Hunter MR,Scourfield EJ,Emmott E,Graham SC

Mon Oct 23 00:00:00 UTC 2017

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

The Biochemical journal

Hunter MR,Scourfield EJ,Emmott E,Graham SC

Mon Oct 23 00:00:00 UTC 2017

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

The Biochemical journal

Hunter MR,Scourfield EJ,Emmott E,Graham SC

Mon Oct 23 00:00:00 UTC 2017

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

The Biochemical journal

Hunter MR,Scourfield EJ,Emmott E,Graham SC

Mon Oct 23 00:00:00 UTC 2017

The second report of a new hypomyelinating disease due to a defect in the VPS11 gene discloses a massive lysosomal involvement.

Journal of inherited metabolic disease

Hörtnagel K,Krägeloh-Mann I,Bornemann A,Döcker M,Biskup S,Mayrhofer H,Battke F,du Bois G,Harzer K

Tue Nov 01 00:00:00 UTC 2016

The second report of a new hypomyelinating disease due to a defect in the VPS11 gene discloses a massive lysosomal involvement.

Journal of inherited metabolic disease

Hörtnagel K,Krägeloh-Mann I,Bornemann A,Döcker M,Biskup S,Mayrhofer H,Battke F,du Bois G,Harzer K

Tue Nov 01 00:00:00 UTC 2016

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.

The Journal of biological chemistry

van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

Fri Dec 18 00:00:00 UTC 2015

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Journal of cell science

van der Kant R,Fish A,Janssen L,Janssen H,Krom S,Ho N,Brummelkamp T,Carette J,Rocha N,Neefjes J

Thu Aug 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: hVPS11; RING finger protein 108; vacuolar protein sorting 11 homolog; Vacuolar protein sorting-associated protein 11 homolog; VPS11 CORVET/HOPS core subunit

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Gene Aliases: 1200011A11Rik; END1; HLD12; hVPS11; PEP5; PP3476; RNF108; VPS11

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UniProt ID: (Human) Q9H270, (Mouse) Q91W86

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Entrez Gene ID: (Human) 55823, (Mouse) 71732, (Rat) 315600

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Function(s)

nucleotide binding protein binding zinc ion binding protein domain specific binding syntaxin binding protein binding, bridging metal ion binding

Process(es)

intracellular protein transport vesicle docking involved in exocytosis autophagy endosome organization lysosome organization endosome to lysosome transport regulation of protein stability endosomal vesicle fusion regulation of SNARE complex assembly regulation of organelle assembly positive regulation of cellular protein catabolic process positive regulation of protein targeting to mitochondrion positive regulation of early endosome to late endosome transport transport protein transport vesicle-mediated transport toxin transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VPS11 Polyclonal Antibody

VPS11 Antibody (19140-1-AP) in ICC/IF

Product Details

19140-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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