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VPS18 Antibody (MA5-37854) in WB
Western blot analysis of VPS18 in extracts of various cell lines. Samples were incubated with VPS18 Monoclonal antibody (Product # MA5-37854) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-37854
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1W8T9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253)
Conjugate
Form
Liquid
Concentration
0.31 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897774
Product Specific Information
Positive Samples: HeLa, Mouse lung, Mouse brain, Rat brain
Immunogen sequence: MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV
Target Information
VPS18 is a vesicle mediated protein that plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hVPS18; vacuolar protein sorting 18 homolog; vacuolar protein sorting protein 18; Vacuolar protein sorting-associated protein 18 homolog; VPS18 CORVET/HOPS core subunit
Gene Aliases: 9930024E13Rik; KIAA1475; PEP3; VPS18
UniProt ID: (Human) Q9P253, (Mouse) Q8R307
Entrez Gene ID: (Human) 57617, (Mouse) 228545, (Rat) 296083
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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