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VPS29 Antibody (BS-12770R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VPS29 Polyclonal Antibody, Unconjugated (bs-12770R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-12770R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human VPS29, amino acids 21-120.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene belongs to a group of vacuolar protein sorting genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp564F0223; FLJ20492; hVPS29; PEP11 homolog; retromer protein; vacuolar protein sorting 29 homolog; Vacuolar protein sorting-associated protein 29; vacuolar sorting protein 29; vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS29 retromer complex component; x 007 protein
Gene Aliases: 2010015D08Rik; AW049835; DC15; DC7; MDS007; PEP11; VPS29
UniProt ID: (Human) Q9UBQ0, (Mouse) Q9QZ88
Entrez Gene ID: (Human) 51699, (Mouse) 56433
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