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VPS33A Antibody (PA5-31456) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of VPS33A was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS33A Polyclonal Antibody (Product # PA5-31456) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-31456
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VPS33A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548930
Product Specific Information
Recommended positive controls: 293T, HeLa, HepG2.
Predicted reactivity: Mouse (97%), Rat (96%), Zebrafish (83%), Xenopus laevis (86%), Pig (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: buff; hVPS33A; vacuolar protein sorting 33 homolog A; vacuolar protein sorting 33A; Vacuolar protein sorting-associated protein 33A; vacuolar sorting protein 33a; VPS33A CORVET/HOPS core subunit
Gene Aliases: 3830421M04Rik; AI503300; AW048546; AW554476; Bf; Buff; VPS33A
UniProt ID: (Human) Q96AX1, (Mouse) Q9D2N9
Entrez Gene ID: (Human) 65082, (Mouse) 77573
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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