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          • Primary Antibodies ›
          • VPS34 Antibodies

          Invitrogen

          VPS34 Polyclonal Antibody

          Advanced Verification
          27 Published Figures
          38 References
          View all (20) VPS34 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite VPS34 Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (27)
          • Advanced Verification (2)
          VPS34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          VPS34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 33

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          VPS34 Antibody (38-2100) in IHC (P)

          Immunohistochemistry analysis of VPS34 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temper... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          VPS34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          VPS34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          VPS34 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          VPS34 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          VPS34 Antibody in Immunohistochemistry (IHC)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Western Blot (WB)
          VPS34 Antibody in Immunoprecipitation (IP)
          VPS34 Antibody
          VPS34 Antibody

          Product Details

          38-2100

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 23 publications 23 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          -

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 6 publications 6 publications

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Hamster, Human, Mouse, Non-human primate, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide derived from the mid region of the human VPS34 protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533360

          Target Information

          PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3

          View more View less

          Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34

          View more View less

          UniProt ID: (Human) Q8NEB9, (Mouse) Q6PF93

          View more View less

          Entrez Gene ID: (Human) 5289, (Mouse) 225326

          View more View less

          Function(s)
          protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor phosphatidylinositol 3-kinase activity
          Process(es)
          autophagosome assembly cytokinesis protein phosphorylation endocytosis autophagy endosome organization cell cycle endosomal transport macroautophagy phosphorylation protein processing pexophagy regulation of cytokinesis phosphatidylinositol-3-phosphate biosynthetic process cellular response to glucose starvation early endosome to late endosome transport phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of protein secretion cell division phosphatidylinositol biosynthetic process toll-like receptor 9 signaling pathway response to leucine
          It has to be done as per old AB suggested Products section.
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