Proteintech

VPS35 Polyclonal Antibody

VPS35 Antibody in Immunocytochemistry (ICC/IF)

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VPS35 Antibody (10236-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10236-1-AP (VPS35 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

VPS35 Antibody in Immunoprecipitation (IP)

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Product Details

10236-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS35 Fusion Protein Ag0340 (1-200 aa encoded by BC002414)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPTTQQSPQ DEQEKLLDEA IQAVKVQSFQ MKRCLDKNKL MDALKHASNM LGELRTSMLS PKSYYELYMA ISDELHYLEV YLTDEFAKGR KVADLYELVQ YAGNIIPRLY LLITVGVVYV KSFPQSRKDI LKDLVEMCRG VQHPLRGLFL RNYLLQCTRN ILPDEGEPTD EETTGDISDS MDFVLLNFAE MNKLWVRMQH Q (1-200 aa encoded by BC002414)

Target Information

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry.

Proceedings of the National Academy of Sciences of the United States of America

Yang B,Jia Y,Meng Y,Xue Y,Liu K,Li Y,Liu S,Li X,Cui K,Shang L,Cheng T,Zhang Z,Hou Y,Yang X,Yan H,Duan L,Tong Z,Wu C,Liu Z,Gao S,Zhuo S,Huang W,Gao GF,Qi J,Shang G

Tue Jan 25 00:00:00 UTC 2022

Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle.

Endocrinology, diabetes & metabolism

Livingstone R,Bryant NJ,Boyle JG,Petrie JR,Gould GW

Thu Sep 01 00:00:00 UTC 2022

Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.

Cell reports

Yong XLH,Zhang L,Yang L,Chen X,Tan JZA,Yu X,Chandra M,Livingstone E,Widagdo J,Vieira MM,Roche KW,Lynch JW,Keramidas A,Collins BM,Anggono V

Tue Jul 06 00:00:00 UTC 2021

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Nature communications

Zhu Y,Feng F,Hu G,Wang Y,Yu Y,Zhu Y,Xu W,Cai X,Sun Z,Han W,Ye R,Qu D,Ding Q,Huang X,Chen H,Xu W,Xie Y,Cai Q,Yuan Z,Zhang R

Thu Feb 11 00:00:00 UTC 2021

Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.

Cell death and differentiation

Lu J,Xu S,Huo Y,Sun D,Hu Y,Wang J,Zhang X,Wang P,Li Z,Liang M,Wu Z,Liu P

Fri Oct 01 00:00:00 UTC 2021

Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.

Cell death and differentiation

Lu J,Xu S,Huo Y,Sun D,Hu Y,Wang J,Zhang X,Wang P,Li Z,Liang M,Wu Z,Liu P

Fri Oct 01 00:00:00 UTC 2021

Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.

Cell death and differentiation

Lu J,Xu S,Huo Y,Sun D,Hu Y,Wang J,Zhang X,Wang P,Li Z,Liang M,Wu Z,Liu P

Fri Oct 01 00:00:00 UTC 2021

Dynein Dysfunction Reproduces Age-Dependent Retromer Deficiency: Concomitant Disruption of Retrograde Trafficking Is Required for Alteration in β-Amyloid Precursor Protein Metabolism.

The American journal of pathology

Kimura N,Samura E,Suzuki K,Okabayashi S,Shimozawa N,Yasutomi Y

Fri Jul 01 00:00:00 UTC 2016

Dynein Dysfunction Reproduces Age-Dependent Retromer Deficiency: Concomitant Disruption of Retrograde Trafficking Is Required for Alteration in β-Amyloid Precursor Protein Metabolism.

The American journal of pathology

Kimura N,Samura E,Suzuki K,Okabayashi S,Shimozawa N,Yasutomi Y

Fri Jul 01 00:00:00 UTC 2016

Dynein Dysfunction Reproduces Age-Dependent Retromer Deficiency: Concomitant Disruption of Retrograde Trafficking Is Required for Alteration in β-Amyloid Precursor Protein Metabolism.

The American journal of pathology

Kimura N,Samura E,Suzuki K,Okabayashi S,Shimozawa N,Yasutomi Y

Fri Jul 01 00:00:00 UTC 2016

Dynein Dysfunction Reproduces Age-Dependent Retromer Deficiency: Concomitant Disruption of Retrograde Trafficking Is Required for Alteration in β-Amyloid Precursor Protein Metabolism.

The American journal of pathology

Kimura N,Samura E,Suzuki K,Okabayashi S,Shimozawa N,Yasutomi Y

Fri Jul 01 00:00:00 UTC 2016

Retromer and Rab2-dependent trafficking mediate PS1 degradation by proteasomes in endocytic disturbance.

Journal of neurochemistry

Ueda N,Tomita T,Yanagisawa K,Kimura N

Sun May 01 00:00:00 UTC 2016

VPS35 pathogenic mutations confer no dominant toxicity but partial loss of function in Drosophila and genetically interact with parkin.

Human molecular genetics

Malik BR,Godena VK,Whitworth AJ

Sun Nov 01 00:00:00 UTC 2015

Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35.

Neural regeneration research

Yan M,Mao S,Dong H,Liu B,Zhang Q,Pan G,Fu Z

Sun Mar 25 00:00:00 UTC 2012

Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35.

Neural regeneration research

Yan M,Mao S,Dong H,Liu B,Zhang Q,Pan G,Fu Z

Sun Mar 25 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35

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Gene Aliases: AI647796; MEM3; PARK17; TCCCTA00141; VPS35

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UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3

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Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114

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Function(s)

protein binding protein transporter activity

Process(es)

intracellular protein transport Wnt signaling pathway regulation of macroautophagy regulation of protein stability retrograde transport, endosome to Golgi transcytosis positive regulation of Wnt protein secretion positive regulation of canonical Wnt signaling pathway negative regulation of late endosome to lysosome transport retrograde transport, endosome to plasma membrane vacuolar protein processing transport protein transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VPS35 Polyclonal Antibody

VPS35 Antibody (10236-1-AP) in ICC/IF

Product Details

10236-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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