Proteintech

VPS4B Polyclonal Antibody

VPS4B Antibody in Immunocytochemistry (ICC/IF)

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VPS4B Antibody (17673-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 17673-1-AP (VPS4B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

VPS4B Antibody in Immunoprecipitation (IP)

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Product Details

17673-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS4B Fusion Protein Ag11610 (1-444 aa encoded by BC039574)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSSTSPNLQ KAIDLASKAA QEDKAGNYEE ALQLYQHAVQ YFLHVVKYEA QGDKAKQSIR AKCTEYLDRA EKLKEYLKNK EKKAQKPVKE GQPSPADEKG NDSDGEGESD DPEKKKLQNQ LQGAIVIERP NVKWSDVAGL EGAKEALKEA VILPIKFPHL FTGKRTPWRG ILLFGPPGTG KSYLAKAVAT EANNSTFFSI SSSDLVSKWL GESEKLVKNL FQLARENKPS IIFIDEIDSL CGSRSENESE AARRIKTEFL VQMQGVGVDN DGILVLGATN IPWVLDSAIR RRFEKRIYIP LPEPHARAAM FKLHLGTTQN SLTEADFREL GRKTDGYSGA DISIIVRDAL MQPVRKVQSA THFKKVRGPS RADPNHLVDD LLTPCSPGDP GAIEMTWMDV PGDKLLEPVV SMSDMLRSLS NTKPTVNEHD LLKLKKFTED FGQEG (1-444 aa encoded by BC039574)

Target Information

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GPR143 controls ESCRT-dependent exosome biogenesis and promotes cancer metastasis.

Developmental cell

Lee YJ,Shin KJ,Jang HJ,Ryu JS,Lee CY,Yoon JH,Seo JK,Park S,Lee S,Je AR,Huh YH,Kong SY,Kwon T,Suh PG,Chae YC

Mon Feb 27 00:00:00 UTC 2023

Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding.

Viruses

Wang Z,Li R,Liu C,Qiao W,Tan J

Thu Mar 03 00:00:00 UTC 2022

RAB31 marks and controls an ESCRT-independent exosome pathway.

Cell research

Wei D,Zhan W,Gao Y,Huang L,Gong R,Wang W,Zhang R,Wu Y,Gao S,Kang T

Mon Feb 01 00:00:00 UTC 2021

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.

Journal of cell science

Parkinson G,Roboti P,Zhang L,Taylor S,Woodman P

Mon Nov 01 00:00:00 UTC 2021

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Journal of extracellular vesicles

Jiang W,Ma P,Deng L,Liu Z,Wang X,Liu X,Long G

Sat Oct 24 00:00:00 UTC 2020

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 UTC 2020

Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.

Science advances

Wang H,Wang X,Zhang K,Wang Q,Cao X,Wang Z,Zhang S,Li A,Liu K,Fang Y

Fri Feb 01 00:00:00 UTC 2019

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

The EMBO journal

Schwenk BM,Hartmann H,Serdaroglu A,Schludi MH,Hornburg D,Meissner F,Orozco D,Colombo A,Tahirovic S,Michaelsen M,Schreiber F,Haupt S,Peitz M,Brüstle O,Küpper C,Klopstock T,Otto M,Ludolph AC,Arzberger T,Kuhn PH,Edbauer D

Wed Nov 02 00:00:00 UTC 2016

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

The EMBO journal

Wed Nov 02 00:00:00 UTC 2016

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

The EMBO journal

Wed Nov 02 00:00:00 UTC 2016

TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.

The EMBO journal

Wed Nov 02 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: cell migration-inducing 1; Cell migration-inducing gene 1 protein; Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B; Vacuolar protein sorting-associated protein 4B

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Gene Aliases: 8030489C12Rik; MIG1; SKD1; SKD1B; VPS4-2; VPS42; VPS4B

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UniProt ID: (Human) O75351, (Mouse) P46467

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Entrez Gene ID: (Human) 9525, (Mouse) 20479, (Rat) 360834

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Function(s)

protein binding ATP binding protein C-terminus binding microtubule-severing ATPase activity ATPase activity ATPase activity, coupled identical protein binding protein homodimerization activity nucleotide binding hydrolase activity molecular_function

Process(es)

cell separation after cytokinesis transport potassium ion transport nucleus organization endosome organization vacuole organization cell cycle mitotic metaphase plate congression regulation of centrosome duplication positive regulation of G2/M transition of mitotic cell cycle protein transport endosomal transport viral release from host cell cholesterol transport endosome to lysosome transport via multivesicular body sorting pathway response to lipid viral budding via host ESCRT complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway positive regulation of viral process regulation of viral process protein depolymerization cell division negative regulation of cell death ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway regulation of mitotic spindle assembly positive regulation of viral release from host cell negative regulation of exosomal secretion positive regulation of exosomal secretion positive regulation of centriole elongation positive regulation of viral life cycle autophagy viral life cycle cytoplasmic microtubule organization multivesicular body assembly late endosomal microautophagy ESCRT III complex disassembly biological_process intracellular cholesterol transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VPS4B Polyclonal Antibody

VPS4B Antibody (17673-1-AP) in ICC/IF

Product Details

17673-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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