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Bethyl Laboratories
VPS4B Polyclonal Antibody
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FIGURE: 1 / 1
VPS4B Antibody (A302-078A) in WB
Detection of human VPS4B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-VPS4B antibody (Product # A302-078A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody A302-077A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 10 seconds (B)... View More
Product Details
A302-078A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 350 and 400 of Human vacuolar protein sorting 4 homolog B.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell migration-inducing 1; Cell migration-inducing gene 1 protein; Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B; Vacuolar protein sorting-associated protein 4B
Gene Aliases: MIG1; SKD1; SKD1B; VPS4-2; VPS42; VPS4B
UniProt ID: (Human) O75351
Entrez Gene ID: (Human) 9525
cell separation after cytokinesis
potassium ion transport
autophagy
nucleus organization
vacuole organization
mitotic metaphase plate congression
regulation of centrosome duplication
positive regulation of G2/M transition of mitotic cell cycle
protein transport
endosomal transport
viral life cycle
viral release from host cell
cholesterol transport
cytoplasmic microtubule organization
endosome to lysosome transport via multivesicular body sorting pathway
response to lipid
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
positive regulation of viral process
regulation of viral process
protein depolymerization
negative regulation of cell death
late endosomal microautophagy
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
negative regulation of exosomal secretion
positive regulation of exosomal secretion
positive regulation of centriole elongation
positive regulation of viral life cycle
ESCRT III complex disassembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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