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Invitrogen
VPS4B Polyclonal Antibody
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FIGURE: 1 / 5
VPS4B Antibody (PA5-30316) in ICC/IF
VPS4B Polyclonal Antibody detects VPS4B protein at nucleus and endosome by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-30316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547790
Product Specific Information
Recommended positive controls: 293T.
Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (88%), Xenopus laevis (88%), Chicken (89%), Rhesus Monkey (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cell migration-inducing 1; Cell migration-inducing gene 1 protein; Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4B; Vacuolar protein sorting-associated protein 4B
Gene Aliases: MIG1; SKD1; SKD1B; VPS4-2; VPS42; VPS4B
UniProt ID: (Human) O75351
Entrez Gene ID: (Human) 9525
cell separation after cytokinesis
potassium ion transport
autophagy
nucleus organization
vacuole organization
mitotic metaphase plate congression
regulation of centrosome duplication
positive regulation of G2/M transition of mitotic cell cycle
protein transport
endosomal transport
viral life cycle
viral release from host cell
cholesterol transport
cytoplasmic microtubule organization
endosome to lysosome transport via multivesicular body sorting pathway
response to lipid
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
positive regulation of viral process
regulation of viral process
protein depolymerization
negative regulation of cell death
late endosomal microautophagy
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
negative regulation of exosomal secretion
positive regulation of exosomal secretion
positive regulation of centriole elongation
positive regulation of viral life cycle
ESCRT III complex disassembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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