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Invitrogen
VRK1 Monoclonal Antibody (1F6)
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FIGURE: 1 / 10
VRK1 Antibody (MA1-828) in ICC/IF
Immunofluorescence analysis of VRK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VRK1 (1F6) Mouse Monoclonal Antibody (Product # MA1-828) at 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
Product Details
MA1-828
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F6
Immunogen
Recombinant fusion protein GST-VRK1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2215678
Product Specific Information
MA1-828 detects VRK1 from human and mouse samples.
MA1-828 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry applications. For Western blotting, it is recommended to block with 3% BSA (not milk).
The MA1-828 immunogen is recombinant fusion protein GST-VRK1.
Target Information
Human vaccinia-related kinase 1 (VRK1) is a new kinase related to a poxvirus kinase, and more distantly to the casein kinase 1 family. VRK1 shows strong autophosphorylating activity in several Ser and Thr residues. VRK has also been found to be an upstream regulator of p53 that belongs to a new signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC117401; MGC138280; Serine/threonine-protein kinase 51PK; Serine/threonine-protein kinase VRK1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1
Gene Aliases: 51PK; PCH1; PCH1A; VRK1
UniProt ID: (Human) Q99986, (Mouse) Q80X41
Entrez Gene ID: (Human) 7443, (Mouse) 22367
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
protein kinase binding
nucleosomal histone binding
histone kinase activity (H3-S10 specific)
histone kinase activity (H3-T3 specific)
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
endocytosis
cell cycle
mitotic nuclear division
regulation of cell shape
Wnt signaling pathway
phosphorylation
peptidyl-serine phosphorylation
histone H3-S10 phosphorylation
protein autophosphorylation
cell division
histone H3-T3 phosphorylation
Golgi disassembly
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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