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Invitrogen
VWF Polyclonal Antibody
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FIGURE: 1 / 2
VWF Antibody (PA5-104687) in ICC/IF
Immunocytochemistry analysis of VWF in HepG2 cells (30 min of 4 µM Forskolin treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with VWF polyclonal antibody (Product # PA5-104687) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blu... View More
Product Details
PA5-104687
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human VWF(Accession P04275), corresponding to amino acid residues N1005-M1055.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853852
Product Specific Information
Antibody detects endogenous levels of total VWF.
Target Information
Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coagulation Factor VIII; coagulation factor VIII VWF; factor viii related antigen; von Willebrand Disease (vWD); von Willebrand factor; vWF; vwf {ECO:0000312|EMBL:CAB37852.1}
Gene Aliases: 6820430P06Rik; AI551257; B130011O06Rik; C630030D09; F8VWF; VWD; VWF
UniProt ID: (Human) P04275, (Mouse) Q8CIZ8
Entrez Gene ID: (Human) 7450, (Mouse) 22371, (Rat) 116669
liver development
placenta development
cell adhesion
blood coagulation
hemostasis
platelet activation
cell-substrate adhesion
protein homooligomerization
response to immobilization stress
tissue regeneration
platelet degranulation
blood coagulation, intrinsic pathway
response to wounding
extracellular matrix organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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