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Exalpha
Vesicle Associated Membrane Protein (VAMP 1/2) Polyclonal Antibody
Product Details
K105P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length VAMP1/2 protein
Conjugate
Form
Liquid
Concentration
0.5-2 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
The antibody may be used in ELISA and IAC. Depending on conjugate: dilution 1/75,000. (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1/75,000 from the delivered solution. Plates are coated with 400 ng/mL BSA-conjugated C6-alkylphenol. HRP-conjugated anti-rabbit IgG as a tracer 1/8,000
Target Information
Vesicle-associated membrane protein 2 is essential for cAMP-regulated exocytosis. VAMP (also know as synaptobrevin) is part of the synaptic vesicle docking and fusion complex and plays a central role in neuroexocytosis. Two VAMP (vesicle-associated membrane protein) isoforms are expressed in the nervous system and are differently distributed among the specialized parts of the tissue. VAMP-1 and -2 are present in all tissues tested, including kidney, adrenal gland, liver, pancreas, thyroid, heart, and smooth muscle. The two isoforms are differentially expressed in various tissues and their level may depend on differentiation. VAMP-1 is restricted to exocrine pancreas and to kidney tubular cells, whereas VAMP-2 is the predominant isoform present in Langerhans islets and in glomerular cells. Both isoforms show a patchy vesicular intracellular distribution in confocal microscopy. Evidence for the importance of neuronal VAMPproteins in the physiology of all cells is indicated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: synaptobrevin 1; synaptobrevin 2; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); Synaptobrevin-1; Synaptobrevin-2; VAMP-1; VAMP-2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 1; vesicle-associated membrane protein 1 (synaptobrevin 1); Vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)
Gene Aliases: RATVAMPB; RATVAMPIR; SPAX1; SYB; SYB1; SYB2; VAMP-1; VAMP-2; VAMP1; VAMP2
UniProt ID: (Human) P23763, (Human) P63027, (Rat) Q63666, (Rat) P63045
Entrez Gene ID: (Human) 6843, (Human) 6844, (Rat) 25624, (Rat) 24803
protein complex assembly
vesicle fusion
neurotransmitter secretion
response to glucose
protein transport
synaptic vesicle exocytosis
vesicle-mediated transport
calcium ion regulated exocytosis
regulation of exocytosis
cellular response to insulin stimulus
Golgi to plasma membrane protein transport
eosinophil degranulation
neutrophil degranulation
natural killer cell degranulation
long-term synaptic potentiation
regulation of vesicle-mediated transport
membrane fusion
mucus secretion
positive regulation of intracellular protein transport
regulation of histamine secretion by mast cell
intracellular protein transport
exocytosis
SNARE complex assembly
post-Golgi vesicle-mediated transport
glutamate secretion
regulation of delayed rectifier potassium channel activity
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