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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Vimentin Antibodies

          Proteintech

          Vimentin Monoclonal Antibody (3H9D1)

          206 References
          View all (199) Vimentin antibodies

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          Datasheet
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          Datasheet
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          Cite Vimentin Monoclonal Antibody (3H9D1)

          • Antibody Testing Data (27)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 27

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          Vimentin Antibody (60330-1-IG) in ICC/IF

          Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 60330-1-IG (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Flow Cytometry (Flow)

          Product Details

          60330-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:8,000
          View 125 publications 125 publications

          Immunohistochemistry (IHC)

          -
          View 20 publications 20 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:2,000-1:10,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 59 publications 59 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat, Zebrafish

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3H9D1

          Immunogen

          Vimentin Fusion Protein Ag0489 (1-466 aa encoded by BC000163)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • CoraLite 488
          • CoraLite 555
          • CoraLite 594
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.8 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MSTRSVSSS SYRRMFGGPG TASRPSSSRS YVTTSTRTYS LGSALRPSTS RSLYASSPGG VYATRSSAVR LRSSVPGVRL LQDSVDFSLA DAINTEFKNT RTNEKVELQE LNDRFANYID KVRFLEQQNK ILLAELEQLK GQGKSRLGDL YEEEMRELRR QVDQLTNDKA RVEVERDNLA EDIMRLREKL QEEMLQREEA ENTLQSFRQD VDNASLARLD LERKVESLQE EIAFLKKLHE EEIQELQAQI QEQHVQIDVD VSKPDLTAAL RDVRQQYESV AAKNLQEAEE WYKSKFADLS EAANRNNDAL RQAKQESTEY RRQVQSLTCE VDALKGTNES LERQMREMEE NFAVEAANYQ DTIGRLQDEI QNMKEEMARH LREYQDLLNV KMALDIEIAT YRKLLEGEES RISLPLPNFS SLNLRETNLD SLPLVDTHSK RTLLIKTVET RDGQVINETS QHHDDLE (1-466 aa encoded by BC000163)

          Target Information

          Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin

          View more View less

          Gene Aliases: CTRCT30; HEL113; VIM

          View more View less

          UniProt ID: (Human) P08670, (Mouse) P20152, (Rat) P31000

          View more View less

          Entrez Gene ID: (Human) 7431, (Mouse) 22352, (Rat) 81818

          View more View less

          Function(s)
          glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding structural molecule activity kinase binding protein kinase binding protein phosphatase 2A binding
          Process(es)
          positive regulation of gene expression negative regulation of neuron projection development astrocyte development intermediate filament-based process intermediate filament organization regulation of axonogenesis Bergmann glial cell differentiation positive regulation of glial cell proliferation SMAD protein signal transduction lens fiber cell development regulation of Schwann cell migration movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization
          It has to be done as per old AB suggested Products section.
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