Invitrogen

Vimentin Monoclonal Antibody (RV202)

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (OMA1-06001) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Vimentin Polyclonal Antibody (Product # OMA1-06001) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

OMA1-06001

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:200

View 7 publications 7 publications

Flow Cytometry (Flow)

1:100-1:200

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish

Published species

Human, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RV202

Immunogen

Cytoskeletal vimentin extract of calf lens.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325529

Product Specific Information

OMA1-06001 detects vimentin from mouse, rat, hamster, chicken, goat, canine, monkey, zebrafish, porcine and human tissues and cells.

OMA1-06001 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects a ~57 kDa protein representing vimentin from human mesenchymal cells.

OMA1-06001 antigen is cytoskeletal vimentin extract of calf lens.

Store at 4ºC or at -20ºC if preferred. Avoid freeze-thaw cycles.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vimentin Localization in the Zebrafish Oral Cavity: A Potential Role in Taste Buds Regeneration.

International journal of molecular sciences

Aragona M,Porcino C,Briglia M,Mhalhel K,Abbate F,Levanti M,Montalbano G,Laurà R,Lauriano ER,Germanà A,Guerrera MC

OMA1-06001 was used in Immunohistochemistry to reveal, for the first time, the presence of Vimentin RV202 in a taste bud cell population of zebrafish.

Thu Oct 26 00:00:00 UTC 2023

Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation.

Scientific reports

Wang M,Silva T,Toothaker JM,McCourt BT,Shugrue C,Desir G,Gorelick F,Konnikova L

Published figure using Vimentin monoclonal antibody (Product # OMA1-06001) in Immunohistochemistry (Paraffin)

Wed Mar 23 00:00:00 UTC 2022

Basic-science observations explain how outer retinal hyperreflective foci predict drusen regression and geographic atrophy in age-related macular degeneration.

Eye (London, England)

Zhang Q,Miller JML

OMA1-06001 was used in Immunocytochemistry-immunofluorescence to show how basic-science observations explain how outer retinal hyperreflective foci predict drusen regression and geographic atrophy in age-related macular degeneration.

Sun May 01 00:00:00 UTC 2022

ROS-Mediated Enamel Formation Disturbance Characterized by Alternative Cervical Loop Cell Proliferation and Downregulation of RhoA/ROCK in Ameloblasts.

Oxidative medicine and cellular longevity

Xu Y,Zhang Y,Zheng J,Xu M,Yang Y,Guo W

OMA1-06001 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate for the first time that SOD1-mediated ROS accumulation disrupted normal enamel structure through alternative cervical loop cell proliferation and downregulation of RhoA and ROCK in ameloblasts, demonstrating the convoluted role of ROS in monitoring the progress of enamel defects.

Fri Oct 28 00:00:00 UTC 2022

Parathyroid hormone-related peptide (1-34) promotes tooth eruption and inhibits osteogenesis of dental follicle cells during tooth development.

Journal of cellular physiology

Zhang J,Liao L,Li Y,Xu Y,Guo W,Tian W,Zou S

OMA1-06001 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to suggest that parathyroid hormone-related peptide (1-34) accelerates tooth eruption and inhibits osteogenesis of dental follicle cells by inactivating Wnt/ß-catenin pathway.

Mon Jul 01 00:00:00 UTC 2019

Effect of canonical NF-κB signaling pathway on the differentiation of rat dental epithelial stem cells.

Stem cell research & therapy

Liang Y,Chen G,Yang Y,Li Z,Chen T,Sun W,Yu M,Pan K,Guo W,Tian W

OMA1-06001 was used in Immunocytochemistry-immunoflourescence to investigate the specific differentiation regulatory mechanisms of the canonical NF-κB signaling pathway in dental epithelial stem cells and provide the mechanism of cross-talk involved in dental epithelial stem cell differentiation.

Mon May 20 00:00:00 UTC 2019

A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein.

Scientific reports

Dusart P,Fagerberg L,Perisic L,Civelek M,Struck E,Hedin U,Uhlén M,Trégouët DA,Renné T,Odeberg J,Butler LM

OMA1-06001 was used in Immunocytochemistry-immunoflourescence to perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status.

Tue Oct 02 00:00:00 UTC 2018

Schwann cells secrete extracellular vesicles to promote and maintain the proliferation and multipotency of hDPCs.

Cell proliferation

Li Z,Liang Y,Pan K,Li H,Yu M,Guo W,Chen G,Tian W

OMA1-06001 was used in Immunocytochemistry-immunoflourescence to study the role of Schwann cell secretion on the development of human dental pulp cells.

Tue Aug 01 00:00:00 UTC 2017

Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway.

Molecular medicine reports

Jiang S,Chen G,Feng L,Jiang Z,Yu M,Bao J,Tian W

OMA1-06001 was used in immunocytochemistry study the role of kif3a in dental mesenchymal stem and precursor cell differentiation

Thu Sep 01 00:00:00 UTC 2016

Osteogenic differentiation and gene expression profile of human dental follicle cells induced by human dental pulp cells.

Journal of molecular histology

Park SJ,Bae HS,Park JC

OMA1-06001 was used in immunocytochemistry to study the effects of dental papilla-derived human dental pulp cells on osteogenic differentiation of human dental follicle cells

Sun Feb 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; intermediate filament; RP11-124N14.1; VIME; Vimentin

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Gene Aliases: BOS_12844; cb28; CTRCT30; HEL113; VIM

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UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3, (Mouse) P20152, (Rat) P31000

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Entrez Gene ID: (Pig) 100522394, (Bovine) 280955, (Chicken) 420519, (Human) 7431, (Dog) 477991, (Mouse) 22352, (Rat) 81818, (Chinese hamster) 100689099, (Zebrafish) 140599

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Function(s)

structural constituent of cytoskeleton structural constituent of eye lens protein C-terminus binding identical protein binding scaffold protein binding structural molecule activity glycoprotein binding double-stranded RNA binding protein binding keratin filament binding kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

positive regulation of gene expression negative regulation of neuron projection development astrocyte development intermediate filament-based process intermediate filament organization regulation of axonogenesis Bergmann glial cell differentiation positive regulation of glial cell proliferation SMAD protein signal transduction lens fiber cell development regulation of Schwann cell migration in utero embryonic development biological_process wound healing movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Vimentin Monoclonal Antibody (RV202)

Vimentin Antibody (OMA1-06001) in ICC/IF

Product Details

OMA1-06001

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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