Invitrogen

Vimentin Monoclonal Antibody (V9), eBioscience™

Vimentin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

Vimentin Antibody (14-9897-82) in ICC/IF

Immunofluorescence analysis of Vimentin was performed using 70 confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Vimentin Monoclonal Antibody (V9), eBioscience™ (Product # 14-9897-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More

Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Vimentin Antibody in Immunohistochemistry (IHC)

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Product Details

14-9897-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 7 publications 7 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Human, Rat

Published species

Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10597910

Product Specific Information

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).

Applications Tested: This V9 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized SK-N-SH or C6 cell lines and by western blot on cell lysates prepared from HeLa cells. This antibody can be used at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Matrix stiffness induces epithelial-to-mesenchymal transition via Piezo1-regulated calcium flux in prostate cancer cells.

iScience

Lopez-Cavestany M,Hahn SB,Hope JM,Reckhorn NT,Greenlee JD,Schwager SC,VanderBurgh JA,Reinhart-King CA,King MR

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Western Blot

Fri Apr 21 00:00:00 UTC 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Western Blot

Mon Mar 20 00:00:00 UTC 2023

Theranostics application of tumor-initiating cell probe TiY in non-small cell lung cancer.

Theranostics

Lee YA,Lek CCJ,Rong G,Wu Z,Shathishwaran S,Lee JHJ,Tam WL,Wuestefeld T,Park SJ,Jung S,Kim B,Kang NY,Chang YT

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Western Blot

Mon Mar 20 00:00:00 UTC 2023

Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition.

Oncology letters

Zhou H,Yun X,Shu Y,Xu K

14-9897-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to reveal that aspirin (ASA) increased the efficacy of gemcitabine in reducing the proliferation, migration and invasion of pancreatic cancer cells and increasing their apoptosis.

Wed Mar 01 00:00:00 UTC 2023

Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition.

Oncology letters

Zhou H,Yun X,Shu Y,Xu K

Wed Mar 01 00:00:00 UTC 2023

Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer.

Frontiers in oncology

Greier MDC,Runge A,Dudas J,Carpentari L,Schartinger VH,Randhawa A,Mayr M,Petersson M,Riechelmann H

14-9897-82 was used in Immunohistochemistry to optimize cultivation conditions for HNC SC and analyze the added effects of platelet rich fibrin (PRF) on these conditions.

Tue Apr 18 00:00:00 UTC 2023

Transcriptome analyses of the cortex and white matter of focal cortical dysplasia type II: Insights into pathophysiology and tissue characterization.

Frontiers in neurology

Assis-Mendonça GR,Athié MCP,Tamanini JVG,de Souza A,Zanetti GG,Araújo PAORA,Ghizoni E,Tedeschi H,Alvim MKM,de Almeida VS,de Souza W,Coras R,Yasuda CL,Blümcke I,Vieira AS,Cendes F,Lopes-Cendes I,Rogerio F

14-9897-82 was used in Immunohistochemistry to evaluate the transcriptomes of the gray and white matters of surgical focal cortical dysplasia type II specimens.

Tue Apr 04 00:00:00 UTC 2023

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

14-9897-82 was used in Immunocytochemistry to identify cell type-specific extracellular vesicle (EV) protein markers in human-induced pluripotent stem cell-derived neural cell types, including excitatory neurons (ATP1A3, NCAM1), astrocytes (LRP1, ITGA6), microglia-like cells (ITGAM, LCP1), and oligodendrocyte-like cells (LAMP2, FTH1), along with 16 pan-EV marker candidates.

Sat Jan 01 00:00:00 UTC 2022

Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.

Journal of nanobiotechnology

Zanjanchi P,Asghari SM,Mohabatkar H,Shourian M,Shafiee Ardestani M

14-9897-82 was used in Western Blot to suggest that GNP-VGB3 is a promising candidate for clinical translation.

Tue Jan 04 00:00:00 UTC 2022

Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity.

Journal of nanobiotechnology

Zanjanchi P,Asghari SM,Mohabatkar H,Shourian M,Shafiee Ardestani M

14-9897-82 was used in Western Blot to suggest that GNP-VGB3 is a promising candidate for clinical translation.

Tue Jan 04 00:00:00 UTC 2022

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Immunocytochemistry

Mon Mar 01 00:00:00 UTC 2021

Characterization of epithelial cells, connective tissue cells and immune cells in human upper airway mucosa by immunofluorescence multichannel image cytometry: a pilot study.

Histochemistry and cell biology

Giotakis AI,Dudas J,Glueckert R,Dejaco D,Ingruber J,Fleischer F,Innerhofer V,Pinggera L,Bektic-Tadic L,Gabriel SAM,Riechelmann H

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Immunocytochemistry

Mon Mar 01 00:00:00 UTC 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

14-9897-82 was used in Western Blot to show that miR-384 targets Smad5 and inactivates the Wnt/β-catenin pathway, which exerts a suppressing role in NPC cell behaviors as well as tumor growth in vivo.

Thu Apr 01 00:00:00 UTC 2021

MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis.

Cytotechnology

Zeng X,Liao H,Wang F

Thu Apr 01 00:00:00 UTC 2021

Induction of Epithelial-mesenchymal Transition in MDCK II Cells.

Bio-protocol

Pastuła A,Lundmark R

14-9897-82 was used in Immunohistochemistry to provide a detailed protocol on how to induce EMT in Madin-Darby Canine Kidney (MDCK) II epithelial cell line and perform immunofluorescent staining on EMT-induced cells.

Fri Feb 05 00:00:00 UTC 2021

Nonmuscle Myosin II Activation Regulates Cell Proliferation, Cell Contraction, and Myofibroblast Differentiation in Keloid-Derived Fibroblasts.

Advances in wound care

Lu YY,Fang CC,Hong CH,Wu CH,Lin YH,Chang KL,Lee CH

14-9897 was used in Immunohistochemistry-immunofluorescence to prove NM-IIA might be one of the therapeutic targets in keloids.

Tue Sep 01 00:00:00 UTC 2020

miR-16 enhances miR-302/367-induced reprogramming and tumor suppression in breast cancer cells.

IUBMB life

Hoseinbeyki M,Taha MF,Javeri A

14-9897-82 was used in Western Blot to study the impact of exogenous induction of miR-16 in MDA-MB-231 and SK-BR-3 breast cancer cells following overexpression of miR-302/367 cluster and investigate whether transfection of these cells by a mature miR-16 mimic could affect the reprogramming state of the cells and their tumorigenicity.

Fri May 01 00:00:00 UTC 2020

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

14-9897 was used in Western Blotting to explore the role of omentin-1 in patient samples with atrial fibrillation.

Wed May 06 00:00:00 UTC 2020

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

14-9897 was used in Western Blotting to explore the role of omentin-1 in patient samples with atrial fibrillation.

Wed May 06 00:00:00 UTC 2020

Omentin-1 is associated with atrial fibrillation in patients with cardiac valve disease.

BMC cardiovascular disorders

Chen Y,Liu F,Han F,Lv L,Tang CE,Xie Z,Luo F

14-9897 was used in Western Blotting to explore the role of omentin-1 in patient samples with atrial fibrillation.

Wed May 06 00:00:00 UTC 2020

GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells.

Stem cell research & therapy

McGrath M,Tam E,Sladkova M,AlManaie A,Zimmer M,de Peppo GM

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Immunocytochemistry

Fri Jan 11 00:00:00 UTC 2019

Levels of hepatic Th17 cells and regulatory T cells upregulated by hepatic stellate cells in advanced HBV-related liver fibrosis.

Journal of translational medicine

Li X,Su Y,Hua X,Xie C,Liu J,Huang Y,Zhou L,Zhang M,Li X,Gao Z

Published figure using Vimentin monoclonal antibody (Product # 14-9897-82) in Immunofluorescence

Tue Apr 11 00:00:00 UTC 2017

Interaction of TWEAK with Fn14 leads to the progression of fibrotic liver disease by directly modulating hepatic stellate cell proliferation.

The Journal of pathology

Wilhelm A,Shepherd EL,Amatucci A,Munir M,Reynolds G,Humphreys E,Resheq Y,Adams DH,Hübscher S,Burkly LC,Weston CJ,Afford SC

14-9897 was used in Immunofluorescence to conclude that TWEAK/Fn14 interaction leads to the progression of fibrotic liver disease via direct modulation of HSCs proliferation, making it a potential therapeutic target for liver fibrosis.

Sun May 01 00:00:00 UTC 2016

Biphasic Squamoid Alveolar Renal Cell Carcinoma: A Distinctive Subtype of Papillary Renal Cell Carcinoma?

The American journal of surgical pathology

Hes O,Condom Mundo E,Peckova K,Lopez JI,Martinek P,Vanecek T,Falconieri G,Agaimy A,Davidson W,Petersson F,Bulimbasic S,Damjanov I,Jimeno M,Ulamec M,Podhola M,Sperga M,Pane Foix M,Shelekhova K,Kalusova K,Hora M,Rotterova P,Daum O,Pivovarcikova K,Michal M

14989782 was used in immunohistochemistry - paraffin section to characterize 21 cases of biphasic squamoid alveolar renal cell carcinoma

Sun May 01 00:00:00 UTC 2016

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

14-9897 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin

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Gene Aliases: CTRCT30; HEL113; VIM

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UniProt ID: (Human) P08670, (Dog) A0A8C0N8E3, (Rat) P31000

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Entrez Gene ID: (Chicken) 420519, (Human) 7431, (Dog) 477991, (Rat) 81818

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Function(s)

structural molecule activity glycoprotein binding double-stranded RNA binding structural constituent of cytoskeleton structural constituent of eye lens protein binding protein C-terminus binding identical protein binding scaffold protein binding keratin filament binding kinase binding protein kinase binding protein phosphatase 2A binding

Process(es)

movement of cell or subcellular component positive regulation of gene expression negative regulation of neuron projection development astrocyte development viral process muscle filament sliding intermediate filament organization Bergmann glial cell differentiation SMAD protein signal transduction lens fiber cell development aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus intermediate filament-based process fat cell differentiation decidualization regulation of axonogenesis positive regulation of glial cell proliferation regulation of Schwann cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Vimentin Monoclonal Antibody (V9), eBioscience™

Vimentin Antibody (14-9897-82) in ICC/IF

Product Details

14-9897-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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