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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Vimentin Antibodies

          Invitrogen

          Vimentin Monoclonal Antibody (V9)

          Advanced Verification
          24 Published Figures
          246 References
          View all (199) Vimentin antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Vimentin Monoclonal Antibody (V9)

          • Antibody Testing Data (15)
          • Published Figures (24)
          • Advanced Verification (4)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 43

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          Vimentin Antibody (MA5-11883) in ICC/IF

          Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin (V9) Mouse Monoclonal Antibody (Product # MA5-11883) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Immunoprecipitation (IP)
          Vimentin Antibody in Immunoprecipitation (IP)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Immunocytochemistry (ICC/IF)
          Vimentin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Vimentin Antibody in Immunohistochemistry (IHC)
          Vimentin Antibody in Immunohistochemistry (IHC)
          Vimentin Antibody in Immunohistochemistry (IHC)
          Vimentin Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Vimentin Antibody in Immunohistochemistry (IHC)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody in Western Blot (WB)
          Vimentin Antibody
          Vimentin Antibody
          Vimentin Antibody
          Vimentin Antibody

          Product Details

          MA5-11883

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 53 publications 53 publications

          Immunohistochemistry (IHC)

          -
          View 129 publications 129 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          View 9 publications 9 publications

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 48 publications 48 publications

          Flow Cytometry (Flow)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Bovine, Dog, Chicken, Horse, Cat, Gerbil, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

          Published species

          Bovine, Cat, Chicken, Dog, Horse, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          V9

          Immunogen

          Purified vimentin from pig eye lens
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 555 
          • Alexa Fluor 647 
          • Alexa Fluor Plus 594 
          • Alexa Fluor Plus 750
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10985392

          Product Specific Information

          MA5-11883 targets Vimentin in IHC (P) applications and shows reactivity with Bovine, Canine, Chicken, Equine, Feline, Gerbil, Hamster, Human, mouse, Non-human primate, Porcine, Rabbit, and Rat samples.

          The MA5-11883 immunogen is purified vimentin from pig eye lens.

          Target Information

          Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin

          View more View less

          Gene Aliases: BOS_12844; CTRCT30; HEL113; VIM

          View more View less

          UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3, (Mouse) P20152, (Rat) P31000

          View more View less

          Entrez Gene ID: (Pig) 100522394, (Bovine) 280955, (Chicken) 420519, (Human) 7431, (Rabbit) 100008924, (Horse) 100056088, (Dog) 477991, (Mouse) 22352, (Rat) 81818, (Chinese hamster) 100689099

          View more View less

          Function(s)
          structural constituent of cytoskeleton structural constituent of eye lens protein C-terminus binding identical protein binding scaffold protein binding structural molecule activity glycoprotein binding double-stranded RNA binding protein binding keratin filament binding kinase binding protein kinase binding protein phosphatase 2A binding
          Process(es)
          positive regulation of gene expression negative regulation of neuron projection development astrocyte development intermediate filament-based process intermediate filament organization regulation of axonogenesis Bergmann glial cell differentiation positive regulation of glial cell proliferation SMAD protein signal transduction lens fiber cell development regulation of Schwann cell migration in utero embryonic development biological_process movement of cell or subcellular component viral process muscle filament sliding aging response to radiation response to zinc ion response to selenium ion response to estradiol response to vitamin D skeletal muscle tissue regeneration cellular response to fibroblast growth factor stimulus fat cell differentiation decidualization
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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