Proteintech

Vinculin Polyclonal Antibody

Vinculin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Vinculin Antibody (26520-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 26520-1-AP) (Vinculin antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

Vinculin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vinculin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

26520-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 31 publications 31 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 21 publications 21 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Vinculin Fusion Protein Ag24942 (777-1066 aa encoded by BC039174)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PKFREAVKA ASDELSKTIS PMVMDAKAVA GNISDPGLQK SFLDSGYRIL GAVAKVREAF QPQEPDFPPP PPDLEQLRLT DELAPPKPPL PEGEVPPPRP PPPEEKDEEF PEQKAGEVIN QPMMMAARQL HDEARKWSSK GNDIIAAAKR MALLMAEMSR LVRGGSGTKR ALIQCAKDIA KASDEVTRLA KEVAKQCTDK RIRTNLLQVC ERIPTISTQL KILSTVKATM LGRTNISDEE SEQATEMLVH NAQNLMQSVK ETVREAEAAS IKIRTDAGFT LRWVRKTPWY Q (777-1066 aa encoded by BC039174)

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: epididymis luminal protein 114; Metavinculin; MV; RP11-178G16.3; vinc; Vinculin

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Gene Aliases: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL

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UniProt ID: (Human) P18206, (Mouse) Q64727

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Entrez Gene ID: (Human) 7414, (Mouse) 22330

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Function(s)

dystroglycan binding actin binding structural molecule activity protein binding beta-catenin binding ubiquitin protein ligase binding alpha-catenin binding cadherin binding cadherin binding involved in cell-cell adhesion Rho GTPase binding actin filament binding

Process(es)

morphogenesis of an epithelium cell adhesion cell-matrix adhesion lamellipodium assembly regulation of cell migration adherens junction assembly protein localization to cell surface apical junction assembly axon extension platelet aggregation epithelial cell-cell adhesion platelet degranulation movement of cell or subcellular component muscle contraction negative regulation of cell migration

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Vinculin Polyclonal Antibody

Vinculin Antibody (26520-1-AP) in ICC/IF

Product Details

26520-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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