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NeoBiotechnologies
Vinculin Monoclonal Antibody (VCL, 2572)
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Product Details
7414-MSM1-P1ABX
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
VCL, 2572
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 114; Metavinculin; MV; RP11-178G16.3; vinc; Vinculin
Gene Aliases: CMD1W; CMH15; HEL114; MV; MVCL; VCL
UniProt ID: (Human) P18206
Entrez Gene ID: (Human) 7414
morphogenesis of an epithelium
platelet degranulation
movement of cell or subcellular component
muscle contraction
cell adhesion
cell-matrix adhesion
lamellipodium assembly
negative regulation of cell migration
adherens junction assembly
protein localization to cell surface
apical junction assembly
axon extension
platelet aggregation
epithelial cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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