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Invitrogen
Vitronectin Monoclonal Antibody (HV2)
FIGURE: 1 / 4
Vitronectin Antibody (CSI 003-02-02) in ICC/IF
Immunofluorescence analysis of Vitronectin was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Anti-Vitronectin Mouse Monoclonal Antibody (Product # CSI-003-0202) at 1:200 dilution in 0.1% BSA, incubated ... View More
Product Details
CSI 003-02-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
HV2
Immunogen
Human vitronectin purified from plasma by heparin-affinity chromatography.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1088278
Product Specific Information
CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse).
CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands.
The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; V75; Vitronectin; VN
Gene Aliases: V75; VN; VNT; VTN
UniProt ID: (Human) P04004
Entrez Gene ID: (Human) 7448
receptor-mediated endocytosis
immune response
cell adhesion
cell-matrix adhesion
positive regulation of cell-substrate adhesion
negative regulation of endopeptidase activity
positive regulation of smooth muscle cell migration
negative regulation of blood coagulation
extracellular matrix organization
regulation of complement activation
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of protein binding
cell adhesion mediated by integrin
endodermal cell differentiation
positive regulation of receptor-mediated endocytosis
oligodendrocyte differentiation
positive regulation of peptidyl-tyrosine phosphorylation
smooth muscle cell-matrix adhesion
positive regulation of wound healing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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