Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
WAC Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
WAC Antibody (PA5-143930) in ICC/IF
Immunocytochemistry analysis of WAC in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in WAC Polyclonal antibody (Product # PA5-143930) using a dilution of 4 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
PA5-143930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human WAC recombinant protein (Position: Q459-V647).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075144
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human THP-1 whole cell, human Raji whole cell, human K562 whole cell, human U20S whole cell, human SK-OV-3 whole cell, rat brain tissue, rat PC-12 whole cell, mouse thymus tissue, mouse SP2/0 whole cell. ICC/IF: A431 cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: WW domain-containing adapter protein with coiled-coil; WW domain-containing adaptor with coiled-coil; WW domain-containing protein 4
Gene Aliases: 1110067P07Rik; A230035H12Rik; AI256735; AI256776; BM-016; DESSH; KIAA1844; PRO1741; RGD1562407; WAC; Wwp4
UniProt ID: (Human) Q9BTA9, (Mouse) Q924H7
Entrez Gene ID: (Human) 51322, (Rat) 307029, (Mouse) 225131
cellular response to DNA damage stimulus
histone monoubiquitination
positive regulation of macroautophagy
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
G1 DNA damage checkpoint
positive regulation of transcription, DNA-templated
histone H2B conserved C-terminal lysine ubiquitination
transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support