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WARS Antibody (PA5-112153) in WB
Western Blot analysis of WARS using WARS Polyclonal Antibody (Product # PA5-112153) at a dilution of 1:500. Lane A: HepG2 whole cell lysate, Lane B: A431 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 55 kDa.
Product Details
PA5-112153
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human WARS/TrpRS protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866889
Product Specific Information
This product can be used in direct ELISA.
Target Information
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hWRS; IFI 53; IFP 53; IFP53; Interferon-induced protein 53; TrpRS; tryptophan tRNA ligase 1, cytoplasmic; Tryptophan--tRNA ligase, cytoplasmic; Tryptophanyl-tRNA synthetase; WARS protein
Gene Aliases: GAMMA-2; IFI53; IFP53; WARS; WARS1; WRS
UniProt ID: (Human) P23381
Entrez Gene ID: (Human) 7453
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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