Proteintech

WASL Polyclonal Antibody

View all (27) N-WASP antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (5)

WASL Antibody in Immunocytochemistry (ICC/IF)

WASL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

WASL Antibody (14306-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using WASL antibody 14306-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

WASL Antibody in Immunocytochemistry (ICC/IF)

WASL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

WASL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

WASL Antibody in Western Blot (WB)

WASL Antibody in Western Blot (WB)

Product Image Gallery

WASL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using WASL antibody 14306-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

WASL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 40x lens).

WASL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 10x lens).

WASL Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WASL Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

14306-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WASL Fusion Protein Ag5424 (1-350 aa encoded by BC052955)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSSVQQQPP PPRRVTNVGS LLLTPQENES LFTFLGKKCV TMSSAVVQLY AADRNCMWSK KCSGVACLVK DNPQRSYFLR IFDIKDGKLL WEQELYNNFV YNSPRGYFHT FAGDTCQVAL NFANEEEAKK FRKAVTDLLG RRQRKSEKRR DPPNGPNLPM ATVDIKNPEI TTNRFYGPQV NNISHTKEKK KGKAKKKRLT KADIGTPSNF QHIGHVGWDP NTGFDLNNLD PELKNLFDMC GISEAQLKDR ETSKVIYDFI EKTGGVEAVK NELRRQAPPP PPPSRGGPPP PPPPPHNSGP PPPPARGRGA PPPPPSRAPT AAPPPPPPSR PSVAVPPPPP NRMYPPPPPA L (1-350 aa encoded by BC052955)

Target Information

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

WASH interacts with Ku to regulate DNA double-stranded break repair.

iScience

Wang T,Du XH,Hong Y,Hong X,Fan L,Zhou JW,Sun H,Ge J,Billadeau DD,Deng ZH

Fri Jan 21 00:00:00 UTC 2022

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Frontiers in immunology

Wang C,Sample KM,Gajendran B,Kapranov P,Liu W,Hu A,Zacksenhaus E,Li Y,Hao X,Ben-David Y

Mon Jul 05 00:00:00 UTC 2021

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Molecular cell

Kovalski JR,Bhaduri A,Zehnder AM,Neela PH,Che Y,Wozniak GG,Khavari PA

Thu Feb 21 00:00:00 UTC 2019

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Molecular cell

Kovalski JR,Bhaduri A,Zehnder AM,Neela PH,Che Y,Wozniak GG,Khavari PA

Thu Feb 21 00:00:00 UTC 2019

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Cellular microbiology

He L,Lin Y,Ge ZH,He SY,Zhao BB,Shen D,He JG,Lu YJ

Sat Jun 01 00:00:00 UTC 2019

Decreased expression of cortactin in the schizophrenia brain.

Neuroreport

Bhambhvani HP,Simmons M,Haroutunian V,Meador-Woodruff JH

Wed Feb 10 00:00:00 UTC 2016

Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.

Journal of dermatological science

Hsu CC,Chow SE,Chen CP,Tsai WC,Wang JS,Yu SY,Lee SC

Sat Jun 01 00:00:00 UTC 2013

Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.

Journal of dermatological science

Hsu CC,Chow SE,Chen CP,Tsai WC,Wang JS,Yu SY,Lee SC

Sat Jun 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Actin nucleation-promoting factor WASL; DKFZp779G0847; MGC48327; n wasp; N-WASP; Neural Wiskott-Aldrich syndrome protein; Wiskott-Aldrich syndrome-like

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Gene Aliases: 2900021I12Rik; 3110031I02Rik; N-WASP; NWASP; TRS4; WASL; WASPB

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UniProt ID: (Human) O00401, (Mouse) Q91YD9

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Entrez Gene ID: (Human) 8976, (Mouse) 73178, (Rat) 682507

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actin binding protein binding GTPase regulator activity identical protein binding

transcription, DNA-templated regulation of transcription, DNA-templated membrane budding actin filament organization cell cycle mitotic nuclear division response to bacterium membrane invagination vesicle organization cell projection assembly actin cytoskeleton organization vesicle transport along actin filament regulation of protein localization cellular protein complex localization actin nucleation cell division positive regulation of filopodium assembly spindle localization membrane tubulation positive regulation of clathrin-mediated endocytosis positive regulation of Arp2/3 complex-mediated actin nucleation negative regulation of membrane tubulation protein complex assembly movement of cell or subcellular component actin polymerization or depolymerization Fc-gamma receptor signaling pathway involved in phagocytosis ephrin receptor signaling pathway regulation of nitric-oxide synthase activity negative regulation of lymphocyte migration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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