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WAVE1 Antibody (PA5-78273) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of WAVE1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WAVE1 Polyclonal Antibody (Product # PA5-78273) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-78273
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse WAVE1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736791
Product Specific Information
Positive Control: mouse brain, rat brain
Predicted Reactivity: Chimpanzee (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin-binding protein WASF1; homology of dictyostelium scar 1; Protein WAVE-1; Verprolin homology domain-containing protein 1; WAS protein family; WAS protein family, member 1; WASP family 1; WASP family protein member 1; Wiskott-Aldrich syndrome protein family member 1
Gene Aliases: AI195380; AI838537; KIAA0269; Scar; SCAR1; WASF1; WAVE; WAVE-1; WAVE1
UniProt ID: (Human) Q92558, (Rat) Q5BJU7, (Mouse) Q8R5H6
Entrez Gene ID: (Human) 8936, (Rat) 294568, (Mouse) 83767
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