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WAVE2 Antibody (MA5-42713) in IHC (P)
Immunohistochemistry analysis of WAVE2 in paraffin-embedded mouse testis. Samples were incubated with WAVE2 Monoclonal antibody (Product # MA5-42713) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-42713
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7A6C10
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 399-498 of human WAVE2/WASF2 (Q9Y6W5).
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911854
Product Specific Information
Positive test controls include: A-549, K-562, Mouse testis, Mouse thymus.
Immunogen sequence: PPGPPPPPFT GADGQPAIPP PLSDTTKPKS SLPAVSDARS DLLSAIRQGF QLRRVEEQRE QEKRDVVGND VATILSRRIA VEYSDSEDDS SEFDEDDWSD
Target Information
WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin-binding protein WASF2; Protein WAVE-2; putative Wiskott-Aldrich syndrome protein family member 4; suppressor of cyclic-AMP receptor (WASP-family); Verprolin homology domain-containing protein 2; WAS protein family, member 2; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin-homologous protein 2; Wiskott-Aldrich syndrome protein family member 2
Gene Aliases: AW742646; D4Ertd13e; dJ393P12.2; IMD2; SCAR2; WASF2; WASF4; WAVE2
UniProt ID: (Human) Q9Y6W5, (Mouse) Q8BH43
Entrez Gene ID: (Human) 10163, (Mouse) 242687, (Rat) 313024
angiogenesis
ameboidal-type cell migration
endocytosis
movement of cell or subcellular component
positive regulation of lamellipodium assembly
Rac protein signal transduction
lamellipodium assembly
actin cytoskeleton organization
actin filament-based movement
megakaryocyte development
negative regulation of stress fiber assembly
lamellipodium morphogenesis
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
viral process
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
cell-cell adhesion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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