Proteintech

WDR24 Polyclonal Antibody

WDR24 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

WDR24 Antibody (20778-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

WDR24 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

WDR24 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20778-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

WDR24 Fusion Protein Ag14774 (570-920 aa encoded by BC008025)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LGRNQVAQT WTMLRIIYCS PGLVPTANLN HSVGKGGSCG LPLMNSFNLK DMAPGLGSET RLDRSKGDAR SDTVLLDSSA TLITNEDNEE TEGSDVPADY LLGDVEGEED ELYLLDPEHA HPEDPECVLP QEAFPLRHEI VDTPPGPEHL QDKADSPHVS GSEADVASLA PVDSSFSLLS VSHALYDSRL PPDFFGVLVR DMLHFYAEQG DVQMAVSVLI VLGERVRKDI DEQTQEHWYT SYIDLLQRFR LWNVSNEVVK LSTSRAVSCL NQASTTLHVN CSHCKRPMSS RGWVCDRCHR CASMCAVCHH VVKGLFVWCQ GCSHGGHLQH IMKWLEGSSH CPAGCGHLCE YS (570-920 aa encoded by BC008025)

Target Information

WDR24, as a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. WDR24 is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1. In addition to its role in regulation of the TORC1 complex, promotes the acidification of lysosomes and facilitates autophagic flux.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of FLCN Phosphorylation in Insulin-Mediated mTORC1 Activation and Tumorigenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang G,Chen L,Lei X,Qin S,Geng H,Zheng Y,Xia C,Yao J,Meng T,Deng L

Thu Jun 01 00:00:00 UTC 2023

Effects of Leucine Ingestion and Contraction on the Sestrin/GATOR2 Pathway and mTORC1 Activation in Rat Fast-Twitch muscle.

The Journal of nutrition

Kanzaki K,Wada M

Tue Aug 01 00:00:00 UTC 2023

Effects of Leucine Ingestion and Contraction on the Sestrin/GATOR2 Pathway and mTORC1 Activation in Rat Fast-Twitch muscle.

The Journal of nutrition

Kanzaki K,Wada M

Tue Aug 01 00:00:00 UTC 2023

Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.

Nature cell biology

Yan G,Yang J,Li W,Guo A,Guan J,Liu Y

Mon May 01 00:00:00 UTC 2023

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.

Cell reports

Yin S,Liu L,Ball LE,Wang Y,Bedford MT,Duncan SA,Wang H,Gan W

Tue Apr 25 00:00:00 UTC 2023

Ring domains are essential for GATOR2-dependent mTORC1 activation.

Molecular cell

Jiang C,Dai X,He S,Zhou H,Fang L,Guo J,Liu S,Zhang T,Pan W,Yu H,Fu T,Li D,Inuzuka H,Wang P,Xiao J,Wei W

Thu Jan 05 00:00:00 UTC 2023

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Nature communications

Chen X,Yu C,Liu X,Liu B,Wu X,Wu J,Yan D,Han L,Tang Z,Yuan X,Wang J,Wang Y,Liu S,Shan L,Shang Y

Thu Dec 08 00:00:00 UTC 2022

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion-positive rhabdomyosarcoma.

JCI insight

Morales J,Allegakoen DV,Garcia JA,Kwong K,Sahu PK,Fajardo DA,Pan Y,Horlbeck MA,Weissman JS,Gustafson WC,Bivona TG,Sabnis AJ

Thu Dec 08 00:00:00 UTC 2022

E3 ligase RNF167 and deubiquitinase STAMBPL1 modulate mTOR and cancer progression.

Molecular cell

Wang D,Xu C,Yang W,Chen J,Ou Y,Guan Y,Guan J,Liu Y

Thu Feb 17 00:00:00 UTC 2022

E3 ligase RNF167 and deubiquitinase STAMBPL1 modulate mTOR and cancer progression.

Molecular cell

Wang D,Xu C,Yang W,Chen J,Ou Y,Guan Y,Guan J,Liu Y

Thu Feb 17 00:00:00 UTC 2022

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Science (New York, N.Y.)

Cangelosi AL,Puszynska AM,Roberts JM,Armani A,Nguyen TP,Spinelli JB,Kunchok T,Wang B,Chan SH,Lewis CA,Comb WC,Bell GW,Helman A,Sabatini DM

Fri Jul 01 00:00:00 UTC 2022

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Science (New York, N.Y.)

Cangelosi AL,Puszynska AM,Roberts JM,Armani A,Nguyen TP,Spinelli JB,Kunchok T,Wang B,Chan SH,Lewis CA,Comb WC,Bell GW,Helman A,Sabatini DM

Fri Jul 01 00:00:00 UTC 2022

Structure of the nutrient-sensing hub GATOR2.

Nature

Valenstein ML,Rogala KB,Lalgudi PV,Brignole EJ,Gu X,Saxton RA,Chantranupong L,Kolibius J,Quast JP,Sabatini DM

Fri Jul 01 00:00:00 UTC 2022

Structure of the nutrient-sensing hub GATOR2.

Nature

Valenstein ML,Rogala KB,Lalgudi PV,Brignole EJ,Gu X,Saxton RA,Chantranupong L,Kolibius J,Quast JP,Sabatini DM

Fri Jul 01 00:00:00 UTC 2022

Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR.

MethodsX

Seibert M,Kurrle N,Stolp V,Nürnberger H,Tzschentke S,Börner L,Wempe F,Serve H,Schnütgen F

Fri Dec 02 00:00:00 UTC 2022

Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR.

MethodsX

Seibert M,Kurrle N,Stolp V,Nürnberger H,Tzschentke S,Börner L,Wempe F,Serve H,Schnütgen F

Fri Dec 02 00:00:00 UTC 2022

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Nature

Long L,Wei J,Lim SA,Raynor JL,Shi H,Connelly JP,Wang H,Guy C,Xie B,Chapman NM,Fu G,Wang Y,Huang H,Su W,Saravia J,Risch I,Wang YD,Li Y,Niu M,Dhungana Y,Kc A,Zhou P,Vogel P,Yu J,Pruett-Miller SM,Peng J,Chi H

Wed Dec 01 00:00:00 UTC 2021

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Nature

Wed Dec 01 00:00:00 UTC 2021

SAR1B senses leucine levels to regulate mTORC1 signalling.

Nature

Chen J,Ou Y,Luo R,Wang J,Wang D,Guan J,Li Y,Xia P,Chen PR,Liu Y

Sun Aug 01 00:00:00 UTC 2021

SAR1B senses leucine levels to regulate mTORC1 signalling.

Nature

Chen J,Ou Y,Luo R,Wang J,Wang D,Guan J,Li Y,Xia P,Chen PR,Liu Y

Sun Aug 01 00:00:00 UTC 2021

Evidence for a role for Sestrin1 in mediating leucine-induced activation of mTORC1 in skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Xu D,Shimkus KL,Lacko HA,Kutzler L,Jefferson LS,Kimball SR

Wed May 01 00:00:00 UTC 2019

CRISPR/Cas9-mediated generic protein tagging in mammalian cells.

Methods (San Diego, Calif.)

Thöne FMB,Kurrle NS,von Melchner H,Schnütgen F

Mon Jul 15 00:00:00 UTC 2019

CRISPR/Cas9-mediated generic protein tagging in mammalian cells.

Methods (San Diego, Calif.)

Thöne FMB,Kurrle NS,von Melchner H,Schnütgen F

Mon Jul 15 00:00:00 UTC 2019

Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.

Journal of cell science

Platani M,Samejima I,Samejima K,Kanemaki MT,Earnshaw WC

Tue May 01 00:00:00 UTC 2018

Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.

Journal of cell science

Platani M,Samejima I,Samejima K,Kanemaki MT,Earnshaw WC

Tue May 01 00:00:00 UTC 2018

SZT2 dictates GATOR control of mTORC1 signalling.

Nature

Peng M,Yin N,Li MO

Thu Mar 16 00:00:00 UTC 2017

SZT2 dictates GATOR control of mTORC1 signalling.

Nature

Peng M,Yin N,Li MO

Thu Mar 16 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: GATOR2 complex protein WDR24; WD repeat-containing protein 24

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Gene Aliases: BC037651; C16orf21; JFP7; WDR24

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UniProt ID: (Human) Q96S15, (Mouse) Q8CFJ9

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Entrez Gene ID: (Human) 84219, (Mouse) 268933

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Function(s)

protein binding molecular_function

Process(es)

biological_process regulation of autophagy positive regulation of TOR signaling cellular response to amino acid starvation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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WDR24 Polyclonal Antibody

WDR24 Antibody (20778-1-AP) in ICC/IF

Product Details

20778-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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