Osenses

WFS1 Polyclonal Antibody

Product Details

OSW00002G-500UG

Applications

Tested Dilution

Publications

Western Blot (WB)

10-50 µg/mL

Immunohistochemistry (IHC)

10-50 µg/mL

Miscellaneous PubMed (MISC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the aa 100-200 of mouse WFS1 (Wolframin) conjugated to an immunogenic carrier protein was used as the antigen

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in rat.

Specificity of this antibody: WFS1.

Target Information

WFS1 is a novel component of Wolfram syndrome, a rare form of juvenile diabetes. WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. It is normally up-regulated during insulin secretion, whereas inactivation of the protein can cause ER stress. Chronic ER stress is a major involvement in Wolfram syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.

Molecular & cellular proteomics : MCP

Munton RP,Tweedie-Cullen R,Livingstone-Zatchej M,Weinandy F,Waidelich M,Longo D,Gehrig P,Potthast F,Rutishauser D,Gerrits B,Panse C,Schlapbach R,Mansuy IM

Thu Feb 01 00:00:00 UTC 2007

WFS1 (Wolfram syndrome 1) gene product: predominant subcellular localization to endoplasmic reticulum in cultured cells and neuronal expression in rat brain.

Human molecular genetics

Takeda K,Inoue H,Tanizawa Y,Matsuzaki Y,Oba J,Watanabe Y,Shinoda K,Oka Y

Thu Mar 01 00:00:00 UTC 2001

A gene encoding a transmembrane protein is mutated in patients with diabetes mellitus and optic atrophy (Wolfram syndrome).

Nature genetics

Inoue H,Tanizawa Y,Wasson J,Behn P,Kalidas K,Bernal-Mizrachi E,Mueckler M,Marshall H,Donis-Keller H,Crock P,Rogers D,Mikuni M,Kumashiro H,Higashi K,Sobue G,Oka Y,Permutt MA

Thu Oct 01 00:00:00 UTC 1998

Diabetes insipidus, diabetes mellitus, optic atrophy and deafness (DIDMOAD) caused by mutations in a novel gene (wolframin) coding for a predicted transmembrane protein.

Human molecular genetics

Strom TM,Hörtnagel K,Hofmann S,Gekeler F,Scharfe C,Rabl W,Gerbitz KD,Meitinger T

Tue Dec 01 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: Wolfram antibody; Wolfram syndrome 1 homolog; Wolfram syndrome 1 protein homolog; Wolframin

View more View less

Gene Aliases: AI481085; Wfs1; wolframin

View more View less

UniProt ID: (Mouse) P56695

View more View less

Entrez Gene ID: (Mouse) 22393, (Rat) 83725

View more View less

Function(s)

calmodulin binding ubiquitin protein ligase binding activating transcription factor binding calcium-dependent protein binding ATPase binding transporter activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter kidney development renal water homeostasis visual perception sensory perception of sound ER-associated ubiquitin-dependent protein catabolic process endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination endoplasmic reticulum calcium ion homeostasis response to endoplasmic reticulum stress glucose homeostasis negative regulation of programmed cell death negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process positive regulation of growth protein stabilization neurological system process positive regulation of protein metabolic process regulation of cell cycle positive regulation of calcium ion transport calcium ion homeostasis negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of ATF6-mediated unfolded protein response negative regulation of type B pancreatic cell apoptotic process pancreas development olfactory behavior positive regulation of proteolysis polyubiquitinated misfolded protein transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

WFS1 Polyclonal Antibody

Product Details

OSW00002G-500UG

Western Blot (WB)

Immunohistochemistry (IHC)

Miscellaneous PubMed (MISC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!