Invitrogen

WIPF1 Polyclonal Antibody

WIPF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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WIPF1 Antibody (PA5-51995) in IHC (P)

Immunohistochemical analysis of WIPF1 in human testis using WIPF1 Polyclonal Antibody (Product # PA5-51995) shows no cytoplasmic positivity in cells in seminiferous ducts as expected.

WIPF1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

WIPF1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of WIPF1 demonstrates antibody specificity. Immunohistochemical analysis of WIPF1 using anti-WIPF1 Polyclonal Antibody (Product # PA5-51995), shows significant staining of WIPF1 in human spleen and shows minimal or weak staining in human pancreas tissues. The relative expression levels of WIPF1 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-51995

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human WIPF1. Recombinant protein control fragment (Product #RP-102120).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2649649

Product Specific Information

Immunogen sequence: PPPPVSRNGS TSRALPATPQ LPSRSGVDSP RSGPRPPLPP DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA RNESRSGSNR RERGAP

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 90%.

Target Information

This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Frontiers in immunology

Wang C,Sample KM,Gajendran B,Kapranov P,Liu W,Hu A,Zacksenhaus E,Li Y,Hao X,Ben-David Y

PA5-51995 was used in Western Blotting to identify FLI1 as a direct transcriptional regulator of WAS and its binding partner WIP.

Mon Jul 05 00:00:00 UTC 2021

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Frontiers in immunology

Wang C,Sample KM,Gajendran B,Kapranov P,Liu W,Hu A,Zacksenhaus E,Li Y,Hao X,Ben-David Y

PA5-51995 was used in Western Blotting to identify FLI1 as a direct transcriptional regulator of WAS and its binding partner WIP.

Mon Jul 05 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: MGC111041; Protein PRPL-2; testicular tissue protein Li 226; WAS/WASL interacting protein family, member 1; WAS/WASL-interacting protein family member 1; WASP-interacting protein; Wiskott-Aldrich syndrome protein interacting protein; Wiskott-Aldrich syndrome protein-interacting protein

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Gene Aliases: PRPL-2; WAS2; WASPIP; WIP; WIPF1

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UniProt ID: (Human) O43516

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Entrez Gene ID: (Human) 7456

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Function(s)

actin binding protein binding profilin binding actin filament binding

Process(es)

actin cortical patch assembly protein complex assembly endocytosis actin polymerization or depolymerization regulation of cell shape actin filament-based movement Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of actin nucleation actin cortical patch localization response to other organism

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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WIPF1 Polyclonal Antibody

WIPF1 Antibody (PA5-51995) in IHC (P)

Product Details

PA5-51995

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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