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Invitrogen
WNK1 Recombinant Rabbit Monoclonal Antibody (6C5S6)
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FIGURE: 1 / 1
WNK1 Antibody (MA5-35466) in WB
Western blot analysis of WNK1 in mouse kidney. Samples were incubated with WNK1 Monoclonal antibody (Product # MA5-35466) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-35466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6C5S6
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WNK1 (Q9H4A3)
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849367
Product Specific Information
Immunogen sequence: MSGGAAEKQS STPGSLFLSP PAPAPKNGSS SDSSVGEKLG AAAADAVTGR TEEYRRRRHT MDKDSRGAAA TTTTTEHRFF RRSVICDSNA TALELPGLPL
Target Information
WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC163339; MGC163341; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; Serine/threonine-protein kinase WNK1; With no K
Gene Aliases: 6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1; Wnk1
UniProt ID: (Mouse) P83741
Entrez Gene ID: (Mouse) 232341
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase inhibitor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
chloride channel inhibitor activity
potassium channel inhibitor activity
protein kinase binding
phosphatase binding
protein serine/threonine kinase inhibitor activity
positive regulation of systemic arterial blood pressure
protein phosphorylation
ion transport
negative regulation of phosphatase activity
phosphorylation
negative regulation of kinase activity
intracellular signal transduction
protein autophosphorylation
regulation of cellular process
negative regulation of protein serine/threonine kinase activity
negative regulation of pancreatic juice secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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