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          • Primary Antibodies ›
          • WNT4 Antibodies

          Invitrogen

          WNT4 Polyclonal Antibody

          View all (20) WNT4 antibodies

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          Cite WNT4 Polyclonal Antibody

          • Antibody Testing Data (1)
          WNT4 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          WNT4 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          WNT4 Antibody (PA5-96818) in WB

          Western blot analysis of WNT4 in extracts of various cell lines. Samples were incubated with WNT4 Polyclonal antibody (Product # PA5-96818) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          WNT4 Antibody in Western Blot (WB)

          Product Details

          PA5-96818

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 122-351 of human WNT4 (NP_1103882)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.72 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2808620

          Product Specific Information

          Immunogen sequence: FAVTRACSSG ELEKCGCDRT VHGVSPQGFQ WSGCSDNIAY GVAFSQSFVD VRERSKGASS SRALMNLHNN EAGRKAILTH MRVECKCHGV SGSCEVKTCW RAVPPFRQVG HALKEKFDGA TEVEPRRVGS SRALVPRNAQ FKPHTDEDLV YLEPSPDFCE QDMRSGVLGT RGRTCNKTSK AIDGCELLCC GRGFHTAQVE LAERCSCKFH WCCFVKCRQC QRLVELHTCR; Positive Samples: A-549, MCF7; Cellular Location: extracellular matrix, extracellular space

          Target Information

          The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Protein Wnt-4; signal molecule; wingless-related MMTV integration site 4; wingless-type MMTV integration site family member 4; wingless-type MMTV integration site family, member 4

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          Gene Aliases: SERKAL; UNQ426/PRO864; WNT-4; WNT4

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          UniProt ID: (Human) P56705, (Rat) Q9QXQ5, (Mouse) P22724

          View more View less

          Entrez Gene ID: (Human) 54361, (Rat) 84426, (Mouse) 22417

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          Function(s)
          transcription corepressor activity frizzled binding receptor agonist activity molecular_function receptor binding protein binding
          Process(es)
          metanephros development branching involved in ureteric bud morphogenesis kidney development mesonephros development epithelial to mesenchymal transition embryonic epithelial tube formation liver development androgen biosynthetic process gamete generation sex differentiation male gonad development female gonad development cellular response to starvation negative regulation of gene expression negative regulation of steroid biosynthetic process Wnt signaling pathway protein palmitoylation regulation of cell-cell adhesion cell differentiation neuron differentiation female sex determination adrenal gland development negative regulation of cell migration positive regulation of bone mineralization positive regulation of aldosterone biosynthetic process response to estradiol positive regulation of collagen biosynthetic process T cell differentiation in thymus immature T cell proliferation in thymus tube morphogenesis non-canonical Wnt signaling pathway non-canonical Wnt signaling pathway via MAPK cascade negative regulation of fibroblast growth factor receptor signaling pathway hormone metabolic process cell fate commitment negative regulation of cell differentiation positive regulation of osteoblast differentiation positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated oocyte development branching morphogenesis of an epithelial tube anatomical structure development smooth muscle cell differentiation positive regulation of stress fiber assembly positive regulation of focal adhesion assembly canonical Wnt signaling pathway somatotropin secreting cell differentiation thyroid-stimulating hormone-secreting cell differentiation mesenchymal to epithelial transition tertiary branching involved in mammary gland duct morphogenesis kidney morphogenesis negative regulation of wound healing mammary gland epithelium development positive regulation of dermatome development paramesonephric duct development negative regulation of testicular blood vessel morphogenesis cellular response to estradiol stimulus cellular response to hydrostatic pressure cellular response to transforming growth factor beta stimulus nephron development renal vesicle formation renal vesicle induction mesonephric tubule development metanephric tubule formation metanephric nephron development metanephric nephron morphogenesis establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway negative regulation of male gonad development positive regulation of cortisol biosynthetic process negative regulation of testosterone biosynthetic process negative regulation of apoptotic signaling pathway positive regulation of GTPase activity metanephric mesenchymal cell differentiation signal transduction cell-cell signaling multicellular organism development
          It has to be done as per old AB suggested Products section.
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