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WNT4 Antibody (710889) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of Wnt4 using Anti-Wnt4 Recombinant Rabbit Superclonal™ Antibody (Product # 710889, 0.5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Wnt4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c... View More
Product Details
710889
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
9HCLC
Immunogen
Multiple peptides corresponding to Human Wnt4 (aa 58-71; 235-253; 258-269)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532778
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat and Mouse.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-4; wingless-type MMTV integration site family member 4; wingless-type MMTV integration site family, member 4
Gene Aliases: SERKAL; UNQ426/PRO864; WNT-4; WNT4
UniProt ID: (Human) P56705
Entrez Gene ID: (Human) 54361
branching involved in ureteric bud morphogenesis
kidney development
epithelial to mesenchymal transition
embryonic epithelial tube formation
liver development
androgen biosynthetic process
male gonad development
female gonad development
cellular response to starvation
negative regulation of gene expression
negative regulation of steroid biosynthetic process
Wnt signaling pathway
regulation of cell-cell adhesion
neuron differentiation
female sex determination
adrenal gland development
negative regulation of cell migration
positive regulation of bone mineralization
positive regulation of aldosterone biosynthetic process
positive regulation of collagen biosynthetic process
immature T cell proliferation in thymus
non-canonical Wnt signaling pathway via MAPK cascade
negative regulation of fibroblast growth factor receptor signaling pathway
positive regulation of GTPase activity
cell fate commitment
negative regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of meiotic nuclear division
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
oocyte development
smooth muscle cell differentiation
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
canonical Wnt signaling pathway
somatotropin secreting cell differentiation
thyroid-stimulating hormone-secreting cell differentiation
mesenchymal to epithelial transition
tertiary branching involved in mammary gland duct morphogenesis
negative regulation of wound healing
mammary gland epithelium development
positive regulation of dermatome development
paramesonephric duct development
negative regulation of testicular blood vessel morphogenesis
cellular response to transforming growth factor beta stimulus
renal vesicle formation
renal vesicle induction
metanephric mesenchymal cell differentiation
metanephric tubule formation
metanephric nephron morphogenesis
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
negative regulation of male gonad development
positive regulation of cortisol biosynthetic process
negative regulation of testosterone biosynthetic process
negative regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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