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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • WNT5A/WNT5B Antibodies

          Proteintech

          WNT5A/B Polyclonal Antibody

          43 References

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite WNT5A/B Polyclonal Antibody

          • Antibody Testing Data (13)
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          WNT5A/B Antibody (55184-1-AP) in IHC (P)

          Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 55184-1-AP) (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          WNT5A/B Antibody in Western Blot (WB)
          WNT5A/B Antibody in Western Blot (WB)
          WNT5A/B Antibody in Western Blot (WB)

          Product Details

          55184-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 27 publications 27 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          -
          View 7 publications 7 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Chicken, Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.37 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Target Information

          The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Protein Wnt-5a; Protein Wnt-5b; wingless-related MMTV integration site 5A; wingless-related MMTV integration site 5B; wingless-type MMTV integration site family member 5A; wingless-type MMTV integration site family member 5B; wingless-type MMTV integration site family, member 5A; wingless-type MMTV integration site family, member 5B; WNT-5A protein; WNT-5B protein

          View more View less

          Gene Aliases: 8030457G12Rik; AW545702; hWNT5A; Wnt-5a; Wnt-5b; WNT5A; WNT5B

          View more View less

          UniProt ID: (Human) P41221, (Human) Q9H1J7, (Mouse) P22725, (Mouse) P22726

          View more View less

          Entrez Gene ID: (Human) 7474, (Human) 81029, (Mouse) 22418, (Mouse) 22419

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding frizzled binding frizzled-2 binding receptor tyrosine kinase-like orphan receptor binding cytokine activity protein binding protein domain specific binding transcription regulatory region DNA binding receptor agonist activity chemoattractant activity involved in axon guidance receptor binding
          Process(es)
          activation of MAPK activity establishment of planar polarity somitogenesis epithelial to mesenchymal transition positive regulation of endothelial cell proliferation heart looping positive regulation of mesenchymal cell proliferation lens development in camera-type eye positive regulation of cytokine secretion involved in immune response type B pancreatic cell development planar cell polarity pathway involved in axis elongation optic cup formation involved in camera-type eye development Wnt signaling pathway, calcium modulating pathway activation of JUN kinase activity axon guidance synapse assembly hindgut morphogenesis midgut development male gonad development anterior/posterior axis specification, embryo response to organic substance positive regulation of endothelial cell migration positive regulation of peptidyl-threonine phosphorylation positive regulation of T cell chemotaxis positive regulation of neuron projection development Wnt signaling pathway olfactory bulb interneuron development neuron differentiation keratinocyte differentiation negative regulation of BMP signaling pathway positive regulation of cGMP metabolic process prostate gland development positive regulation of protein binding activation of protein kinase B activity response to estradiol positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of peptidyl-serine phosphorylation response to testosterone cellular protein localization non-canonical Wnt signaling pathway post-anal tail morphogenesis chemoattraction of serotonergic neuron axon chemorepulsion of dopaminergic neuron axon non-canonical Wnt signaling pathway via JNK cascade negative regulation of fibroblast growth factor receptor signaling pathway wound healing embryonic digit morphogenesis positive regulation of macrophage activation negative regulation of apoptotic process estrous cycle positive regulation of chemokine biosynthetic process cell fate commitment establishment of epithelial cell apical/basal polarity negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of angiogenesis positive regulation of ossification positive regulation of endocytosis positive regulation of meiotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of melanin biosynthetic process positive regulation of fibroblast proliferation lung alveolus development paraxial mesoderm formation notochord morphogenesis embryonic skeletal system development genitalia development negative regulation of axon extension involved in axon guidance hypophysis morphogenesis negative regulation of epithelial cell proliferation positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of NF-kappaB transcription factor activity cartilage development response to glucocorticoid negative regulation of synapse assembly response to hyperoxia palate development convergent extension involved in axis elongation convergent extension involved in organogenesis uterus development cervix development vagina development canonical Wnt signaling pathway Wnt signaling pathway, planar cell polarity pathway urinary bladder development face development positive regulation of type I interferon-mediated signaling pathway lateral sprouting involved in mammary gland duct morphogenesis mesenchymal-epithelial cell signaling negative regulation of prostatic bud formation mammary gland branching involved in thelarche epithelial cell proliferation involved in mammary gland duct elongation positive regulation of response to cytokine stimulus regulation of branching involved in mammary gland duct morphogenesis planar cell polarity pathway involved in gastrula mediolateral intercalation mesodermal to mesenchymal transition involved in gastrulation positive regulation of macrophage cytokine production positive regulation of cartilage development planar cell polarity pathway involved in outflow tract morphogenesis planar cell polarity pathway involved in ventricular septum morphogenesis planar cell polarity pathway involved in cardiac right atrium morphogenesis planar cell polarity pathway involved in cardiac muscle tissue morphogenesis planar cell polarity pathway involved in pericardium morphogenesis positive regulation of thymocyte apoptotic process cellular response to lipopolysaccharide cellular response to calcium ion cellular response to retinoic acid cellular response to interferon-gamma hematopoietic stem cell proliferation cellular response to transforming growth factor beta stimulus negative regulation of mesenchymal cell proliferation primitive streak formation positive regulation of protein kinase C signaling negative regulation of canonical Wnt signaling pathway cochlea morphogenesis planar cell polarity pathway involved in neural tube closure activation of GTPase activity melanocyte proliferation positive regulation of protein kinase C activity positive regulation of protein localization to synapse regulation of cellular protein localization excitatory synapse assembly inhibitory synapse assembly negative regulation of cell proliferation in midbrain Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation planar cell polarity pathway involved in midbrain dopaminergic neuron differentiation positive regulation of cell-cell adhesion mediated by cadherin positive regulation of non-canonical Wnt signaling pathway positive regulation of interleukin-8 secretion chondrocyte differentiation positive regulation of cell migration fat cell differentiation positive regulation of fat cell differentiation lens fiber cell development signal transduction cell-cell signaling multicellular organism development organ morphogenesis ameboidal-type cell migration positive regulation of cytokine production neural tube closure positive regulation of protein phosphorylation morphogenesis of an epithelium pericardium morphogenesis axis elongation protein phosphorylation JNK cascade determination of left/right symmetry positive regulation of cell proliferation embryo development anterior/posterior pattern specification cell migration neural tube development positive regulation of cell-cell adhesion cell differentiation lung development embryonic limb morphogenesis regulation of protein localization limb morphogenesis inner ear morphogenesis positive regulation of JUN kinase activity positive regulation of JNK cascade development of primary male sexual characteristics digestive tract morphogenesis neuron projection morphogenesis positive regulation of epithelial cell proliferation negative chemotaxis convergent extension tube closure cellular response to molecule of bacterial origin dopaminergic neuron differentiation
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