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Invitrogen
WNT6 Polyclonal Antibody
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WNT6 Antibody (PA5-47580) in IHC (P)
Immunohistochemical analysis of WNT6 in immersion fixed paraffin-embedded sections of human colon cancer tissue. Samples were incubated in WNT6 polyclonal antibody (Product # PA5-47580) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47580
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human Wnt-6 Met35-Glu75 and Gln238-Arg299
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576272
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-6; wingless-type MMTV integration site family member 6; wingless-type MMTV integration site family, member 6
Gene Aliases: WNT6
UniProt ID: (Human) Q9Y6F9
Entrez Gene ID: (Human) 7475
branching involved in ureteric bud morphogenesis
axis specification
positive regulation of gene expression
Wnt signaling pathway
neuron differentiation
odontogenesis of dentin-containing tooth
cell fate commitment
positive regulation of transcription, DNA-templated
epithelial-mesenchymal cell signaling
cornea development in camera-type eye
positive regulation of tooth mineralization
cellular response to retinoic acid
nephron tubule formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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