Invitrogen

WNT7A/WNT7B Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-47430

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human Wnt-7a Leu32-Glu72, Thr216-Arg292
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610494

Product Specific Information

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt-16 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Wnt proteins constitute a family of secreted glycoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Protein Wnt-7a; Protein Wnt-7b; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family member 7A; wingless-type MMTV integration site family member 7B; wingless-type MMTV integration site family, member 7A; wingless-type MMTV integration site family, member 7B

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Gene Aliases: WNT7A; WNT7B

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UniProt ID: (Human) O00755, (Human) P56706

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Entrez Gene ID: (Human) 7476, (Human) 7477

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Function(s)
receptor binding frizzled binding cytokine activity protein binding receptor agonist activity
Process(es)
embryonic axis specification cartilage condensation angiogenesis chondrocyte differentiation neurotransmitter secretion axonogenesis synapse assembly sex differentiation asymmetric protein localization dorsal/ventral pattern formation positive regulation of endothelial cell migration skeletal muscle satellite cell activation skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Wnt signaling pathway cerebellar granule cell differentiation cell proliferation in forebrain central nervous system vasculogenesis establishment of cell polarity regulation of axon diameter response to estradiol somatic stem cell population maintenance embryonic forelimb morphogenesis embryonic hindlimb morphogenesis non-canonical Wnt signaling pathway Wnt signaling pathway involved in wound healing, spreading of epidermal cells synaptic vesicle recycling embryonic digit morphogenesis negative regulation of apoptotic process response to estrogen cell fate commitment positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade somatic stem cell division stem cell development negative regulation of neurogenesis positive regulation of synapse assembly palate development positive regulation of epithelial cell proliferation involved in wound healing oviduct development canonical Wnt signaling pathway dendritic spine morphogenesis uterus morphogenesis lens fiber cell development cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway positive regulation of protein localization to synapse excitatory synapse assembly positive regulation of excitatory synapse assembly positive regulation of excitatory postsynaptic potential in utero embryonic development metanephros morphogenesis establishment or maintenance of polarity of embryonic epithelium forebrain regionalization neuron differentiation lung development oxygen homeostasis homeostatic process cellular metabolic process positive regulation of osteoblast differentiation fibroblast proliferation embryonic organ development synapse organization lung morphogenesis lung epithelium development lobar bronchus development trachea cartilage morphogenesis developmental growth involved in morphogenesis embryonic placenta morphogenesis chorio-allantoic fusion mammary gland epithelium development cellular response to retinoic acid renal inner medulla development renal outer medulla development outer medullary collecting duct development inner medullary collecting duct development stem cell proliferation metanephric collecting duct development metanephric epithelium development metanephric loop of Henle development
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