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Invitrogen
WT1-AS Polyclonal Antibody
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FIGURE: 1 / 2
WT1-AS Antibody (PA5-103316) in IHC (P)
Immunohistochemistry analysis of WT1-AS in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with WT1-AS polyclonal antibody (Product # PA5-103316) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WT1-AS(Accession Q06250), corresponding to amino acid residues V12-E62.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852661
Product Specific Information
Antibody detects endogenous levels of total WIT1.
Target Information
Wilms' tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms' tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms' tumors. Wilms' tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms' tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene Aliases: 6030498F17; AI314831; WIT-1; WIT1; Wt1-as; WT1-AS1; WT1AS
Entrez Gene ID: (Human) 51352, (Mouse) 329480
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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