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Invitrogen
WWC1 Polyclonal Antibody
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FIGURE: 1 / 4
WWC1 Antibody (PA5-143889) in ICC/IF
Immunocytochemistry analysis of WWC1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in WWC1 Polyclonal antibody (Product # PA5-143889) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
PA5-143889
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human KIBRA/WWC1 recombinant protein (Position: D990-Q1080).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075103
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Mcf-7 whole cell, human MDA-MB-453 whole cell, human HEK293 whole cell, rat brain tissue, mouse brain tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
WWC1 (Protein KIBRA) is a probable regulator of the Hippo/SWH signaling pathway. This pathway plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. WWC1, along with NF2, can synergistically induce the phosphorylation of LATS1 and LATS2. It acts as a transcriptional coactivator of ESR1 - which plays an essential role in DYNLL1-mediated ESR1 transactivation. WWC1 also functions as a regulatior of collagen-stimulated activation of the ERK/MAPK cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10865; FLJ23369; HBeAg-binding protein 3; KIBRA; Kidney and brain protein; Protein KIBRA; protein phosphatase 1, regulatory subunit 168; protein WWC1; WW and C2 domain containing 1; WW domain-containing protein 1; WW, C2 and coiled-coil domain containing 1
Gene Aliases: AA408228; AU017197; BC037006; HBEBP3; HBEBP36; KIAA0869; KIBRA; MEMRYQTL; mKIAA0869; PPP1R168; RGD1308329; WWC1
UniProt ID: (Human) Q8IX03, (Mouse) Q8VD17
Entrez Gene ID: (Human) 23286, (Rat) 303039, (Mouse) 211652
negative regulation of transcription from RNA polymerase II promoter
cell migration
regulation of hippo signaling
negative regulation of hippo signaling
positive regulation of MAPK cascade
negative regulation of organ growth
transcription, DNA-templated
establishment of cell polarity
regulation of intracellular transport
hippo signaling
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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