NeoBiotechnologies

Wilms Tumor 1 (WT1) Recombinant Mouse Monoclonal Antibody (rWT1, 857)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

7490-MSM6-P1ABX

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL
-

Peptide Array (Array)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

rWT1, 857

Immunogen

Recombinant full-length human WT1 protein
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Wilms tumor protein; Wilms tumor protein homolog; Wilms tumor protein isoform Ex4a(+); WT; WT33

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Gene Aliases: AWT1; D630046I19Rik; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; Wt-1; WT1; WT33

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UniProt ID: (Human) P19544, (Rat) P49952

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Entrez Gene ID: (Human) 7490, (Mouse) 22431, (Rat) 24883

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Function(s)
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding C2H2 zinc finger domain binding nucleic acid binding DNA binding double-stranded DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter vasculogenesis metanephros development ureteric bud development branching involved in ureteric bud morphogenesis kidney development mesonephros development regulation of organ formation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process germ cell development spermatogenesis single fertilization thorax and anterior abdomen determination heart development sex determination negative regulation of cell proliferation RNA splicing gonad development male gonad development tissue development regulation of gene expression negative regulation of translation negative regulation of cell growth sperm motility adrenal gland development male genitalia development epithelial cell differentiation glomerulus development glomerular basement membrane development adrenal cortex formation camera-type eye development positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter Sertoli cell development mesenchymal to epithelial transition positive regulation of heart growth diaphragm development cardiac muscle cell fate commitment coronary vasculature development visceral serous pericardium development cellular response to cAMP cellular response to gonadotropin stimulus metanephric mesenchyme development glomerular visceral epithelial cell differentiation posterior mesonephric tubule development metanephric S-shaped body morphogenesis negative regulation of metanephric glomerular mesangial cell proliferation seminiferous tubule development negative regulation of mesenchymal cell apoptotic process involved in metanephros development positive regulation of male gonad development negative regulation of female gonad development positive regulation of metanephric ureteric bud development metanephric epithelium development
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