Invitrogen

XBP1 Polyclonal Antibody

View all (26) XBP1 antibodies

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Product Details

PA5-20218

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. Amino acid range: 40-90.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153798

Product Specific Information

A suggested positive control is HepG2 cell lysate.

PA5-20218 can be used with blocking peptide PEP-0351.

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

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Gene Aliases: D11Ertd39e; TREB-5; TREB5; XBP-1; XBP1; XBP2

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UniProt ID: (Human) P17861, (Mouse) O35426

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Entrez Gene ID: (Human) 7494, (Mouse) 22433

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
angiogenesis liver development endothelial cell proliferation positive regulation of immunoglobulin production transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process fatty acid biosynthetic process autophagy immune response positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response muscle organ development regulation of autophagy negative regulation of myotube differentiation protein transport endoplasmic reticulum unfolded protein response exocrine pancreas development protein destabilization cellular response to nutrient cellular response to insulin stimulus cellular triglyceride homeostasis IRE1-mediated unfolded protein response ATF6-mediated unfolded protein response cellular response to glucose starvation cholesterol homeostasis negative regulation of apoptotic process positive regulation of MHC class II biosynthetic process positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter neuron development positive regulation of immunoglobulin secretion fatty acid homeostasis sterol homeostasis adipose tissue development epithelial cell maturation involved in salivary gland development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to fructose stimulus cellular response to glucose stimulus cellular response to interleukin-4 cellular response to peptide hormone stimulus positive regulation of plasma cell differentiation positive regulation of endoplasmic reticulum unfolded protein response positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of lactation response to insulin-like growth factor stimulus positive regulation of hepatocyte proliferation positive regulation of interleukin-6 secretion negative regulation of transcription from RNA polymerase II promoter positive regulation of protein phosphorylation epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter lipid metabolic process transport apoptotic process response to unfolded protein multicellular organism development positive regulation of autophagy phosphatidylinositol 3-kinase signaling cell growth cell differentiation positive regulation of histone methylation regulation of protein stability positive regulation of TOR signaling cellular response to oxidative stress response to endoplasmic reticulum stress cellular response to vascular endothelial growth factor stimulus response to drug glucose homeostasis positive regulation of transcription factor import into nucleus vascular endothelial growth factor receptor signaling pathway response to electrical stimulus serotonin secretion, neurotransmission cellular response to fluid shear stress cellular response to laminar fluid shear stress negative regulation of endoplasmic reticulum unfolded protein response positive regulation of protein acetylation positive regulation of endothelial cell apoptotic process
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