Invitrogen

XPG Monoclonal Antibody (8H7)

Product Details

MA1-26186

Applications

Tested Dilution

Publications

Western Blot (WB)

1:625-1:1250

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Immunoprecipitation (IP)

2 µg/mg protein lysate

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

8H7

Immunogen

Recombinant human XPG (Xeroderma Pigmentosum type G) protein produced in Baculovirus.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2098158

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair.

Nature communications

van Cuijk L,van Belle GJ,Turkyilmaz Y,Poulsen SL,Janssens RC,Theil AF,Sabatella M,Lans H,Mailand N,Houtsmuller AB,Vermeulen W,Marteijn JA

MA1-26186 was used in immunocytochemistry to elucidate ubiquitylation of XPC

Tue Jul 07 00:00:00 UTC 2015

Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.

DNA repair

Zhu Q,Wani G,Sharma N,Wani A

MA1-26186 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair

Sat Dec 01 00:00:00 UTC 2012

p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG.

DNA repair

Tillhon M,Cazzalini O,Nardo T,Necchi D,Sommatis S,Stivala LA,Scovassi AI,Prosperi E

MA1-26186 was used in western blot to study the acetylation of XPG by p300/CBP acetyl transferases and the potential modulatory role of PCNA-p21

Mon Oct 01 00:00:00 UTC 2012

Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.

PloS one

Arab HH,Wani G,Ray A,Shah ZI,Zhu Q,Wani AA

MA1-26186 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH

Tue Jun 08 00:00:00 UTC 2010

Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair.

Nucleic acids research

Cazzalini O,Perucca P,Savio M,Necchi D,Bianchi L,Stivala LA,Ducommun B,Scovassi AI,Prosperi E

MA1-26186 was used in western blot to study the role of the cell cycle inhibitor p21(CDKN1A) in regulating nucleotide excision repair

Sat Mar 01 00:00:00 UTC 2008

Molecular characterization of spontaneous mesenchymal stem cell transformation.

PloS one

Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A

MA1-26186 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

Wed Jan 02 00:00:00 UTC 2008

Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen.

Journal of cell science

Perucca P,Cazzalini O,Mortusewicz O,Necchi D,Savio M,Nardo T,Stivala LA,Leonhardt H,Cardoso MC,Prosperi E

MA1-26186 was used in immunocytochemistry to study the dynamics of cyclin-dependent kinase inhibitor p21CDKN1A recruitment to DNA-damage sites

Sat Apr 15 00:00:00 UTC 2006

Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.

Molecular and cellular biology

Zotter A,Luijsterburg MS,Warmerdam DO,Ibrahim S,Nigg A,van Cappellen WA,Hoeijmakers JH,van Driel R,Vermeulen W,Houtsmuller AB

MA1-26186 was used in immunocytochemistry to study the role of TFIIH in the function of nucleotide excision repair endonuclease XPG

Fri Dec 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: COFS 3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; ERCC 5; ERCM 2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; OTTHUMP00000064902; RP11-484I6.5; Xe; xeroderma pigmentosum; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPG; XPG-complementing protein

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Gene Aliases: COFS3; ERCC5; ERCC5-201; ERCM2; UVDR; XPG; XPGC

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UniProt ID: (Human) P28715

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Entrez Gene ID: (Human) 2073

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Function(s)

bubble DNA binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding protein homodimerization activity metal ion binding protein N-terminus binding

Process(es)

transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion response to UV UV protection response to UV-C nucleotide-excision repair, DNA incision negative regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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XPG Monoclonal Antibody (8H7)

Product Details

MA1-26186

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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