Invitrogen

YAP1 Recombinant Rabbit Monoclonal Antibody (5J9N6)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-51328

Applications
Tested Dilution
Publications

Western Blot (WB)

1:20,000-1:80,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-

ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5J9N6

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094113

Product Specific Information

Immunogen sequence: PTAQHLRQSS FEIPDDVPLP AGWEMAKTSS GQRYFLNHID QTTTWQDPRK AMLSQMNVTA PTSPPVQQNM MNSASGPLPD GWEQAMTQDG EIYYINHKNK TTSWLDPRLD PRFAMNQRIS QSAPVKQPPP LAPQSPQGGV MGGSNSNQQQ QMRLQQLQME KERLRLKQQE LLRQAMRNIN PSTANSPKCQ ELALRSQLPT LEQDGGTQNP VSSPGMSQEL RTMTTNSSDP FLNSGTYHSR DESTDSGLSM SSYSVPRTPD DFLNSVDEMD TGDTINQSTL PSQQNRFPDY LEAIPGTNVD LGTLEGDGMN IEGEELMPSL QEALSSDILN DMESVLAATK LDKESFLTWL

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

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Gene Aliases: AI325207; COB1; YAP; YAP1; YAP2; YAP65; YKI; Yorkie

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UniProt ID: (Human) P46937, (Mouse) P46938

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Entrez Gene ID: (Human) 10413, (Rat) 363014, (Mouse) 22601

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Function(s)
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding chromatin binding transcription coactivator activity transcription corepressor activity protein binding protein C-terminus binding transcription regulatory region DNA binding proline-rich region binding
Process(es)
cell morphogenesis vasculogenesis embryonic heart tube morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular response to DNA damage stimulus DNA damage induced protein phosphorylation cell proliferation positive regulation of cell proliferation regulation of keratinocyte proliferation keratinocyte differentiation negative regulation of epithelial cell differentiation notochord development somatic stem cell population maintenance hippo signaling regulation of cell proliferation positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth paraxial mesoderm development lateral mesoderm development contact inhibition bud elongation involved in lung branching lung epithelial cell differentiation regulation of canonical Wnt signaling pathway cellular response to retinoic acid cellular response to gamma radiation regulation of stem cell proliferation regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of canonical Wnt signaling pathway positive regulation of stem cell population maintenance negative regulation of nucleic acid-templated transcription negative regulation of stem cell differentiation negative regulation of extrinsic apoptotic signaling pathway regulation of neurogenesis transcription initiation from RNA polymerase II promoter
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