Proteintech

YBX1 Polyclonal Antibody

YBX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

YBX1 Antibody (20339-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 20339-1-AP (YBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

YBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

YBX1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20339-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 10 publications 10 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

YBX1 Fusion Protein Ag14129 (134-324 aa encoded by BC010430)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QGSKYAADR NHYRRYPRRR GPPRNYQQNY QNSESGEKNE GSESAPEGQA QQRRPYRRRR FPPYYMRRPY GRRPQYSNPP VQGEVMEGAD NQGAGEQGRP VRQNMYRGYR PRFRRGPPRQ RQPREDGNEE DKENQGDETQ GQQPPQRRYR RNFNYRRRRP ENPKPQDGKE TKAADPPAEN SSAPEAEQGG AE (134-324 aa encoded by BC010430)

Target Information

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing.

Frontiers in oncology

Vital T,Wali A,Butler KV,Xiong Y,Foster JP,Marcel SS,McFadden AW,Nguyen VU,Bailey BM,Lamb KN,James LI,Frye SV,Mosely AL,Jin J,Pattenden SG,Davis IJ

Mon Apr 22 00:00:00 UTC 2024

Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos.

Journal of cellular physiology

Jiang WJ,Sun MH,Li XH,Lee SH,Heo G,Zhou D,Cui XS

Sat Jul 01 00:00:00 UTC 2023

Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos.

Journal of cellular physiology

Jiang WJ,Sun MH,Li XH,Lee SH,Heo G,Zhou D,Cui XS

Sat Jul 01 00:00:00 UTC 2023

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Cell reports

Curdy N,Lanvin O,Cerapio JP,Pont F,Tosolini M,Sarot E,Valle C,Saint-Laurent N,Lhuillier E,Laurent C,Fournié JJ,Franchini DM

Tue Mar 28 00:00:00 UTC 2023

Long non-coding RNA KCND1 protects hearts from hypertrophy by targeting YBX1.

Cell death & disease

Yang R,Li L,Hou Y,Li Y,Zhang J,Yang N,Zhang Y,Ji W,Yu T,Lv L,Liang H,Li X,Li T,Shan H

Tue May 30 00:00:00 UTC 2023

LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.

Journal of experimental & clinical cancer research : CR

Li D,Shen L,Zhang X,Chen Z,Huang P,Huang C,Qin S

Fri Dec 02 00:00:00 UTC 2022

YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer.

Cell death and differentiation

Jiang D,Qiu T,Peng J,Li S,Ren W,Yang C,Wen Y,Chen CH,Sun J,Wu Y,Liu R,Zhou J,Wu K,Liu W,Mao X,Zhou Z,Chen C

Wed Jun 01 00:00:00 UTC 2022

TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis.

Biochimica et biophysica acta. Molecular basis of disease

Guo Y,Zhu J,Xu X,Shen B,Shen Z,Li B,Li F,Gu T,Cai X,Dong H,Lu L

Sat Jan 01 00:00:00 UTC 2022

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.

Science advances

Zhang C,Wang M,Li Y,Zhang Y

Fri Feb 04 00:00:00 UTC 2022

Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1.

Cell reports

Avolio R,Inglés-Ferrándiz M,Ciocia A,Coll O,Bonnin S,Guitart T,Ribó A,Gebauer F

Tue Jan 11 00:00:00 UTC 2022

Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1.

Cell reports

Avolio R,Inglés-Ferrándiz M,Ciocia A,Coll O,Bonnin S,Guitart T,Ribó A,Gebauer F

Tue Jan 11 00:00:00 UTC 2022

Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.

Journal of experimental & clinical cancer research : CR

Li C,Wang W,Sun Y,Ni Y,Qin F,Li X,Wang T,Guo M,Sun G

Mon Apr 11 00:00:00 UTC 2022

CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1.

Journal of translational medicine

Lin Y,Zhang J,Li Y,Guo W,Chen L,Chen M,Chen X,Zhang W,Jin X,Jiang M,Xiao H,Wang C,Song C,Fu F

Thu Jan 06 00:00:00 UTC 2022

The long noncoding RNA AATBC promotes breast cancer migration and invasion by interacting with YBX1 and activating the YAP1/Hippo signaling pathway.

Cancer letters

Wang M,Dai M,Wang D,Tang T,Xiong F,Xiang B,Zhou M,Li X,Li Y,Xiong W,Li G,Zeng Z,Guo C

Sun Aug 01 00:00:00 UTC 2021

The long noncoding RNA AATBC promotes breast cancer migration and invasion by interacting with YBX1 and activating the YAP1/Hippo signaling pathway.

Cancer letters

Wang M,Dai M,Wang D,Tang T,Xiong F,Xiang B,Zhou M,Li X,Li Y,Xiong W,Li G,Zeng Z,Guo C

Sun Aug 01 00:00:00 UTC 2021

The long noncoding RNA AATBC promotes breast cancer migration and invasion by interacting with YBX1 and activating the YAP1/Hippo signaling pathway.

Cancer letters

Wang M,Dai M,Wang D,Tang T,Xiong F,Xiang B,Zhou M,Li X,Li Y,Xiong W,Li G,Zeng Z,Guo C

Sun Aug 01 00:00:00 UTC 2021

hTERT Promotes CRC Proliferation and Migration by Recruiting YBX1 to Increase NRF2 Expression.

Frontiers in cell and developmental biology

Gong C,Yang H,Wang S,Liu J,Li Z,Hu Y,Chen Y,Huang Y,Luo Q,Wu Y,Liu E,Xiao Y

Sat Jun 05 00:00:00 UTC 2021

hTERT Promotes CRC Proliferation and Migration by Recruiting YBX1 to Increase NRF2 Expression.

Frontiers in cell and developmental biology

Gong C,Yang H,Wang S,Liu J,Li Z,Hu Y,Chen Y,Huang Y,Luo Q,Wu Y,Liu E,Xiao Y

Sat Jun 05 00:00:00 UTC 2021

Effects of Y Box Binding Protein-1 in Progression and Prognosis of Cholangiocarcinoma.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

Chen Y,Jiang P,Tian F,Chen G,Peng T,Deng X,Li Z,Huang D,Zhu J,Zhen P,Xie C,Wang S

Fri Jan 01 00:00:00 UTC 2021

Effects of Y Box Binding Protein-1 in Progression and Prognosis of Cholangiocarcinoma.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

Chen Y,Jiang P,Tian F,Chen G,Peng T,Deng X,Li Z,Huang D,Zhu J,Zhen P,Xie C,Wang S

Fri Jan 01 00:00:00 UTC 2021

Effects of Y Box Binding Protein-1 in Progression and Prognosis of Cholangiocarcinoma.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

Chen Y,Jiang P,Tian F,Chen G,Peng T,Deng X,Li Z,Huang D,Zhu J,Zhen P,Xie C,Wang S

Fri Jan 01 00:00:00 UTC 2021

Long noncoding RNA SNHG6 promotes carcinogenesis by enhancing YBX1-mediated translation of HIF1α in clear cell renal cell carcinoma.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhao P,Deng Y,Wu Y,Guo Q,Zhou L,Yang X,Wang C

Mon Feb 01 00:00:00 UTC 2021

A reciprocal feedback of miR-548ac/YB-1/Snail induces EndMT of HUVECs during acidity microenvironment.

Cancer cell international

Chen J,Han S,Chen J,Hu P,Zeng Z,Hu Y,Xiong H,Ke Z,Zhang Y,Xu F,Zhao G

Mon Dec 20 00:00:00 UTC 2021

Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis.

Systems biology in reproductive medicine

Liang J,Zheng Y,Zeng W,Chen L,Yang S,Du P,Wang Y,Yu X,Zhang X

Thu Apr 01 00:00:00 UTC 2021

Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis.

Systems biology in reproductive medicine

Liang J,Zheng Y,Zeng W,Chen L,Yang S,Du P,Wang Y,Yu X,Zhang X

Thu Apr 01 00:00:00 UTC 2021

Proteomic Profile of Sperm in Infertile Males Reveals Changes in Metabolic Pathways.

The protein journal

Liang J,Zheng Y,Zeng W,Chen L,Yang S,Du P,Wang Y,Yu X,Zhang X

Wed Dec 01 00:00:00 UTC 2021

Proteomic Profile of Sperm in Infertile Males Reveals Changes in Metabolic Pathways.

The protein journal

Liang J,Zheng Y,Zeng W,Chen L,Yang S,Du P,Wang Y,Yu X,Zhang X

Wed Dec 01 00:00:00 UTC 2021

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Nature communications

Lei H,Xu HZ,Shan HZ,Liu M,Lu Y,Fang ZX,Jin J,Jing B,Xiao XH,Gao SM,Gao FH,Xia L,Yang L,Liu LG,Wang WW,Liu CX,Tong Y,Wu YZ,Zheng JK,Chen GQ,Zhou L,Wu YL

Mon Jan 04 00:00:00 UTC 2021

Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX_36-58.

Annals of translational medicine

Wang X,Liu G,Sheng N,Zhang M,Pan X,Liu S,Huang K,Cong Y,Xu Q,Jia X,Xu J

Sat Aug 01 00:00:00 UTC 2020

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Molecular therapy : the journal of the American Society of Gene Therapy

Ruan H,Song Z,Cao Q,Ni D,Xu T,Wang K,Bao L,Tong J,Xiao H,Xiao W,Cheng G,Xiong Z,Liang H,Liu D,Wang L,Olivier T,Jane BH,Yang H,Zhang X,Chen K

Wed May 06 00:00:00 UTC 2020

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Molecular therapy : the journal of the American Society of Gene Therapy

Ruan H,Song Z,Cao Q,Ni D,Xu T,Wang K,Bao L,Tong J,Xiao H,Xiao W,Cheng G,Xiong Z,Liang H,Liu D,Wang L,Olivier T,Jane BH,Yang H,Zhang X,Chen K

Wed May 06 00:00:00 UTC 2020

Enhanced YB1/EphA2 axis signaling promotes acquired resistance to sunitinib and metastatic potential in renal cell carcinoma.

Oncogene

Ruan H,Li S,Bao L,Zhang X

Tue Sep 01 00:00:00 UTC 2020

Y-box binding protein 1 acts as a negative regulator of stearoyl CoA desaturase 1 in clear cell renal cell carcinoma.

Oncology letters

Jeffords E,Freeman S,Cole B,Root K,Chekouo T,Melvin RG,Bemis L,Simmons GE

Sun Nov 01 00:00:00 UTC 2020

LncRNA LINC00472 regulates cell stiffness and inhibits the migration and invasion of lung adenocarcinoma by binding to YBX1.

Cell death & disease

Deng X,Xiong W,Jiang X,Zhang S,Li Z,Zhou Y,Xiang B,Zhou M,Li X,Li G,Zeng Z,Gong Z

Tue Nov 03 00:00:00 UTC 2020

LncRNA LINC00472 regulates cell stiffness and inhibits the migration and invasion of lung adenocarcinoma by binding to YBX1.

Cell death & disease

Deng X,Xiong W,Jiang X,Zhang S,Li Z,Zhou Y,Xiang B,Zhou M,Li X,Li G,Zeng Z,Gong Z

Tue Nov 03 00:00:00 UTC 2020

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Nature

An H,Ordureau A,Körner M,Paulo JA,Harper JW

Wed Jul 01 00:00:00 UTC 2020

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Molecular metabolism

Zhang W,Dong Z,Xu M,Zhang S,Liu C,Chen S

Sat Feb 01 00:00:00 UTC 2020

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Molecular metabolism

Zhang W,Dong Z,Xu M,Zhang S,Liu C,Chen S

Sat Feb 01 00:00:00 UTC 2020

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA-Binding Proteins and Sponging MiR-942.

Cancer research

Wong CH,Lou UK,Li Y,Chan SL,Tong JH,To KF,Chen Y

Mon Jun 01 00:00:00 UTC 2020

Targeting the YB-1/PD-L1 Axis to Enhance Chemotherapy and Antitumor Immunity.

Cancer immunology research

Tao Z,Ruan H,Sun L,Kuang D,Song Y,Wang Q,Wang T,Hao Y,Chen K

Mon Jul 01 00:00:00 UTC 2019

Targeting the YB-1/PD-L1 Axis to Enhance Chemotherapy and Antitumor Immunity.

Cancer immunology research

Tao Z,Ruan H,Sun L,Kuang D,Song Y,Wang Q,Wang T,Hao Y,Chen K

Mon Jul 01 00:00:00 UTC 2019

PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.

Journal of experimental & clinical cancer research : CR

Hou P,Chen F,Yong H,Lin T,Li J,Pan Y,Jiang T,Li M,Chen Y,Song J,Zheng J,Bai J

Wed Jul 10 00:00:00 UTC 2019

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Cell reports

Shelkovnikova TA,An H,Skelt L,Tregoning JS,Humphreys IR,Buchman VL

Tue Dec 24 00:00:00 UTC 2019

Stress granules regulate stress-induced paraspeckle assembly.

The Journal of cell biology

An H,Tan JT,Shelkovnikova TA

Mon Dec 02 00:00:00 UTC 2019

Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis.

Theranostics

He X,Wang S,Li M,Zhong L,Zheng H,Sun Y,Lai Y,Chen X,Wei G,Si X,Han Y,Huang S,Li X,Liao W,Liao Y,Bin J

Wed Sep 09 00:00:00 UTC 2020

YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.

Nucleic acids research

Lyons SM,Achorn C,Kedersha NL,Anderson PJ,Ivanov P

Fri Aug 19 00:00:00 UTC 2016

YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.

Nucleic acids research

Lyons SM,Achorn C,Kedersha NL,Anderson PJ,Ivanov P

Fri Aug 19 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y box transcription factor; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1

View more View less

Gene Aliases: 1700102N10Rik; BP-8; Byb1; C79409; CBF-A; Cbfa; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1

View more View less

UniProt ID: (Human) P67809, (Mouse) P62960, (Rat) P62961

View more View less

Entrez Gene ID: (Human) 4904, (Mouse) 22608, (Rat) 500538

View more View less

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding poly(A) RNA binding GTPase binding p53 binding nucleic acid binding chromatin binding mRNA binding transcription factor binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing positive regulation of cell proliferation RNA splicing negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway negative regulation of striated muscle cell differentiation positive regulation of cell division CRD-mediated mRNA stabilization mRNA splicing, via spliceosome protein localization to cytoplasmic stress granule

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

YBX1 Polyclonal Antibody

YBX1 Antibody (20339-1-AP) in ICC/IF

Product Details

20339-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!