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Invitrogen
YOD1 Polyclonal Antibody
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FIGURE: 1 / 1
YOD1 Antibody (PA5-89126) in WB
Western blot analysis of YOD1 in K-562 cells. Samples were incubated with YOD1 Polyclonal antibody (Product # PA5-89126) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-89126
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human YOD1 (NP_0610363)
Conjugate
Form
Liquid
Concentration
0.46 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805371
Product Specific Information
Immunogen sequence: MFGPAKGRHF GVHPAPGFPG GVSQQAAGTK AGPAGAWPVG SRTDTMWRLR CKAKDGTHVL QGLSSRTRVR ELQGQIAAIT GIAPGGQRIL VGYPPECLDL SNGDTILEDL PIQSGDMLII EEDQTRPRSS PAFTKRGASS YVRETLPVLT RTVVPADNSC LFTSVYYVVE GGVLNPACAP EMRRLIAQIV ASDPDFYSEA ILGKTNQEYC DWIKRDDTWG GAIEISILSK FYQCEICVVD TQTVRIDRFG EDAGYTKRVL LIYDGIHYDP LQRNFPDPDT PPLTIFSSND DIVLVQALEL ADEARRRRQF TDVNRFTLRC MVCQKGLTGQ AEAREHAKET GHTNFGEV; Positive Samples: Mouse brain
Target Information
Deubiquitinating enzymes (DUBs) are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DUBA-8; HIN-7; HIV-1-induced protease 7; HIV-induced protein-7-like protease; hsHIN7; OTU domain containing 2; OTU domain-containing protein 2; Ubiquitin thioesterase OTU1; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast)
Gene Aliases: 9930028C20Rik; DUBA8; HIN7; Hshin7; OTUD2; PRO0907; YOD1
UniProt ID: (Human) Q5VVQ6, (Mouse) Q8CB27
Entrez Gene ID: (Human) 55432, (Mouse) 226418
thiol-dependent ubiquitin-specific protease activity
protein binding
ubiquitin protein ligase binding
metal ion binding
Lys63-specific deubiquitinase activity
ubiquitin-specific protease activity involved in negative regulation of retrograde protein transport, ER to cytosol
Lys48-specific deubiquitinase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
protein K29-linked deubiquitination
protein K11-linked deubiquitination
protein K63-linked deubiquitination
protein K48-linked deubiquitination
negative regulation of retrograde protein transport, ER to cytosol
protein K27-linked deubiquitination
protein K33-linked deubiquitination
proteolysis
response to unfolded protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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