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FIGURE: 1 / 1
YTHDC1 Antibody (PA5-96634) in WB
Western blot analysis of YTHDC1 in extracts of various cell lines. Samples were incubated with YTHDC1 Polyclonal antibody (Product # PA5-96634) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-96634
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human YTHDC1 (NP_0010269021)
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808436
Product Specific Information
Immunogen sequence: MAADSREEKD GELNVLDDIL TEVPEQDDEL YNPESEQDKN EKKGSKRKSD RMESTDTKRQ KPSVHSRQLV SKPL
Positive Samples: SKOV3, SW620, MCF7, Jurkat, HeLa, Mouse brain, Rat brain; Cellular Location: Nucleus
Target Information
May be part of a signal transduction pathway that influences splice site selection. Target protein binds nuclear transcriptosomal complex scaffold attachment factor B (SAF-B) and Sam68; may play a role in splice site selection and alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: putative splicing factor YT521; RA301-binding protein; Splicing factor YT521; splicing factor YT521-B; YT521-B; YTH domain-containing protein 1
Gene Aliases: A730098D12Rik; C80342; KIAA1966; mKIAA1966; YT521; YT521-B; YTHDC1
UniProt ID: (Human) Q96MU7, (Mouse) E9Q5K9, (Rat) Q9QY02
Entrez Gene ID: (Human) 91746, (Mouse) 231386, (Rat) 170956
Performance Guarantee
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