Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
YY1 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 12
YY1 Antibody (PA5-29171) in ICC/IF
Immunofluorescence analysis of YY1 was performed using DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with YY1 Rabbit Polyclonal Antibody (Product # PA5-29171) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 fo... View More
Product Details
PA5-29171
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human YY1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546647
Product Specific Information
Recommended positive controls: A431, NIH-3T3, PC-12, Rat2.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (98%), Chicken (98%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta transcription factor; INO80 complex subunit S; NF-E1; Transcriptional repressor protein YY1; UCR-motif DNA-binding protein; UCRBP transcription factor; Yin and yang 1; Yin and Yang 1 protein; Yin Yang 1; YY-1
Gene Aliases: AW488674; DELTA; INO80S; NF-E1; NMP-1; NMP1; UCRBP; YIN-YANG-1; YY1
UniProt ID: (Human) P25490, (Mouse) Q00899
Entrez Gene ID: (Human) 7528, (Rat) 24919, (Mouse) 22632
four-way junction DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
enhancer sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
RNA binding
protein binding
zinc ion binding
transcription regulatory region DNA binding
SMAD binding
histone deacetylase binding
sequence-specific DNA binding
metal ion binding
nucleic acid binding
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
RNA localization
cellular response to DNA damage stimulus
spermatogenesis
anterior/posterior pattern specification
response to UV-C
positive regulation of gene expression
cellular response to UV
response to prostaglandin F
positive regulation of transcription from RNA polymerase II promoter
camera-type eye morphogenesis
chromosome organization
cellular response to interleukin-1
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
cell differentiation
DNA repair
DNA recombination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support