Proteintech

ZC3HAV1 Polyclonal Antibody

ZC3HAV1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

ZC3HAV1 Antibody (16820-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 16820-1-AP ( ZC3HAV1 Antibody) at dilution of 1:50 (under 40x lens).

ZC3HAV1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16820-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZC3HAV1 Fusion Protein Ag10364 (1-351 aa encoded by BC025308)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADPEVCCF ITKILCAHGG RMALDALLQE IALSEPQLCE VLQVAGPDRF VVLETGGEAG ITRSVVATTR ARVCRRKYCQ RPCDNLHLCK LNLLGRCNYS QSERNLCKYS HEVLSEENFK VLKNHELSGL NKEELAVLLL QSDPFFMPEI CKSYKGEGRQ QICNQQPPCS RLHICDHFTR GNCRFPNCLR SHNLMDRKVL AIMREHGLNP DVVQNIQDIC NSKHMQKNPP GPRAPSSHRR NMAYRARSKS RDRFFQGSQE FLASASASAE RSCTPSPDQI SHRASLEDAP VDDLTRKFTY LGSQDRARPP SGSSKATDLG GTSQAGTSQR FLENGSQEDL LHGNPGSTYL AS (1-351 aa encoded by BC025308)

Target Information

The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rational attenuation of RNA viruses with zinc finger antiviral protein.

Nature microbiology

Gonçalves-Carneiro D,Mastrocola E,Lei X,DaSilva J,Chan YF,Bieniasz PD

Sat Oct 01 00:00:00 UTC 2022

STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment.

Cancer cell international

Pu Z,Liu J,Liu Z,Peng F,Zhu Y,Wang X,He J,Yi P,Hu X,Fan X,Chen J

Wed Oct 12 00:00:00 UTC 2022

A novel IFNbeta-induced long non-coding RNA ZAP-IT1 interrupts Zika virus replication in A549 cells.

Virologica Sinica

Huang Y,Su Y,Shen L,Huo Z,Chen C,Sun T,Tian X,Li N,Yang C

Thu Dec 01 00:00:00 UTC 2022

HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.

Cell host & microbe

Bou-Nader C,Muecksch F,Brown JB,Gordon JM,York A,Peng C,Ghirlando R,Summers MF,Bieniasz PD,Zhang J

Wed Sep 08 00:00:00 UTC 2021

Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets.

eLife

Rodriguez KM,Buch-Larsen SC,Kirby IT,Siordia IR,Hutin D,Rasmussen M,Grant DM,David LL,Matthews J,Nielsen ML,Cohen MS

Thu Jan 21 00:00:00 UTC 2021

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Nature communications

Zimmer MM,Kibe A,Rand U,Pekarek L,Ye L,Buck S,Smyth RP,Cicin-Sain L,Caliskan N

Fri Dec 10 00:00:00 UTC 2021

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Nature communications

Zimmer MM,Kibe A,Rand U,Pekarek L,Ye L,Buck S,Smyth RP,Cicin-Sain L,Caliskan N

Fri Dec 10 00:00:00 UTC 2021

Origin and evolution of the zinc finger antiviral protein.

PLoS pathogens

Gonçalves-Carneiro D,Takata MA,Ong H,Shilton A,Bieniasz PD

Thu Apr 01 00:00:00 UTC 2021

Development and Validation of Ten-RNA Binding Protein Signature Predicts Overall Survival in Osteosarcoma.

Frontiers in molecular biosciences

Zhang J,Miao X,Wu T,Jia J,Cheng X

Tue Dec 21 00:00:00 UTC 2021

The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral UL4/UL5 Transcripts.

mBio

Gonzalez-Perez AC,Stempel M,Wyler E,Urban C,Piras A,Hennig T,Ganskih S,Wei Y,Heim A,Landthaler M,Pichlmair A,Dölken L,Munschauer M,Erhard F,Brinkmann MM

Tue May 04 00:00:00 UTC 2021

The antiviral state has shaped the CpG composition of the vertebrate interferome to avoid self-targeting.

PLoS biology

Shaw AE,Rihn SJ,Mollentze N,Wickenhagen A,Stewart DG,Orton RJ,Kuchi S,Bakshi S,Collados MR,Turnbull ML,Busby J,Gu Q,Smollett K,Bamford CGG,Sugrue E,Johnson PCD,Da Silva AF,Castello A,Streicker DG,Robertson DL,Palmarini M,Wilson SJ

Wed Sep 01 00:00:00 UTC 2021

Association of Zinc Finger Antiviral Protein Binding to Viral Genomic RNA with Attenuation of Replication of Echovirus 7.

mSphere

Goonawardane N,Nguyen D,Simmonds P

Wed Jan 06 00:00:00 UTC 2021

Association of Zinc Finger Antiviral Protein Binding to Viral Genomic RNA with Attenuation of Replication of Echovirus 7.

mSphere

Goonawardane N,Nguyen D,Simmonds P

Wed Jan 06 00:00:00 UTC 2021

Zinc-finger antiviral protein (ZAP) is a restriction factor for replication of modified vaccinia virus Ankara (MVA) in human cells.

PLoS pathogens

Peng C,Wyatt LS,Glushakow-Smith SG,Lal-Nag M,Weisberg AS,Moss B

Sat Aug 01 00:00:00 UTC 2020

Porcine reproductive and respiratory syndrome virus Nsp4 cleaves ZAP to antagonize its antiviral activity.

Veterinary microbiology

Zhao Y,Song Z,Bai J,Liu X,Nauwynck H,Jiang P

Sun Nov 01 00:00:00 UTC 2020

Zinc Finger CCCH-Type Antiviral Protein 1 Restricts the Viral Replication by Positively Regulating Type I Interferon Response.

Frontiers in microbiology

Zhang B,Goraya MU,Chen N,Xu L,Hong Y,Zhu M,Chen JL

Mon Sep 28 00:00:00 UTC 2020

The role of ZAP and OAS3/RNAseL pathways in the attenuation of an RNA virus with elevated frequencies of CpG and UpA dinucleotides.

Nucleic acids research

Odon V,Fros JJ,Goonawardane N,Dietrich I,Ibrahim A,Alshaikhahmed K,Nguyen D,Simmonds P

Thu Sep 05 00:00:00 UTC 2019

SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.

Cell

Gregersen LH,Mitter R,Ugalde AP,Nojima T,Proudfoot NJ,Agami R,Stewart A,Svejstrup JQ

Thu Jun 13 00:00:00 UTC 2019

CpG-Recoding in Zika Virus Genome Causes Host-Age-Dependent Attenuation of Infection With Protection Against Lethal Heterologous Challenge in Mice.

Frontiers in immunology

Trus I,Udenze D,Berube N,Wheler C,Martel MJ,Gerdts V,Karniychuk U

Wed Nov 11 00:00:00 UTC 2020

ZAP, a CCCH-Type Zinc Finger Protein, Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication and Interacts with Viral Nsp9.

Journal of virology

Zhao Y,Song Z,Bai J,Liu X,Nauwynck H,Jiang P

Wed May 15 00:00:00 UTC 2019

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Nucleic acids research

Booy EP,McRae EK,Ezzati P,Choi T,Gussakovsky D,McKenna SA

Fri Nov 30 00:00:00 UTC 2018

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Nucleic acids research

Booy EP,McRae EK,Ezzati P,Choi T,Gussakovsky D,McKenna SA

Fri Nov 30 00:00:00 UTC 2018

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.

PLoS pathogens

Chiu HP,Chiu H,Yang CF,Lee YL,Chiu FL,Kuo HC,Lin RJ,Lin YL

Sun Jul 01 00:00:00 UTC 2018

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.

PLoS pathogens

Chiu HP,Chiu H,Yang CF,Lee YL,Chiu FL,Kuo HC,Lin RJ,Lin YL

Sun Jul 01 00:00:00 UTC 2018

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.

PLoS pathogens

Chiu HP,Chiu H,Yang CF,Lee YL,Chiu FL,Kuo HC,Lin RJ,Lin YL

Sun Jul 01 00:00:00 UTC 2018

Inhibition of Japanese encephalitis virus infection by the host zinc-finger antiviral protein.

PLoS pathogens

Chiu HP,Chiu H,Yang CF,Lee YL,Chiu FL,Kuo HC,Lin RJ,Lin YL

Sun Jul 01 00:00:00 UTC 2018

A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.

eLife

OhAinle M,Helms L,Vermeire J,Roesch F,Humes D,Basom R,Delrow JJ,Overbaugh J,Emerman M

Thu Dec 06 00:00:00 UTC 2018

A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.

eLife

OhAinle M,Helms L,Vermeire J,Roesch F,Humes D,Basom R,Delrow JJ,Overbaugh J,Emerman M

Thu Dec 06 00:00:00 UTC 2018

CG dinucleotide suppression enables antiviral defence targeting non-self RNA.

Nature

Takata MA,Gonçalves-Carneiro D,Zang TM,Soll SJ,York A,Blanco-Melo D,Bieniasz PD

Thu Oct 05 00:00:00 UTC 2017

CG dinucleotide suppression enables antiviral defence targeting non-self RNA.

Nature

Takata MA,Gonçalves-Carneiro D,Zang TM,Soll SJ,York A,Blanco-Melo D,Bieniasz PD

Thu Oct 05 00:00:00 UTC 2017

RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.

BMC biology

Choudhury NR,Heikel G,Trubitsyna M,Kubik P,Nowak JS,Webb S,Granneman S,Spanos C,Rappsilber J,Castello A,Michlewski G

Wed Nov 08 00:00:00 UTC 2017

RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.

BMC biology

Choudhury NR,Heikel G,Trubitsyna M,Kubik P,Nowak JS,Webb S,Granneman S,Spanos C,Rappsilber J,Castello A,Michlewski G

Wed Nov 08 00:00:00 UTC 2017

Zinc finger antiviral protein inhibits coxsackievirus B3 virus replication and protects against viral myocarditis.

Antiviral research